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Chromogenic platform based on recombinant Drosophila melanogaster acetylcholinesterase for visible unidirectional assay of organophosphate and carbamate insecticide residues

文献类型： 外文期刊

作者： Han, Zheng 1 ; Chi, Chensen 2 ; Bai, Bing 1 ; Liu, Gang 1 ; Rao, Qinxiong 1 ; Peng, Shaojie 3 ; Liu, Hong 4 ; Zhao, Zhihu 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai 201403, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Bor Luh Food Safety Ctr, Shanghai 200240, Peoples R China

3.Inst Shanghai Food & Drug Supervis, Shanghai 200233, Peoples R China

4.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China

关键词： Chromogenic platform;Visible unidirectional assay;Insecticides;On-site analysis

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

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收录情况： SCI

摘要： In this study we propose a chromogenic platform for rapid analysis of organophosphate (OP) and carbamate (CM) insecticide residues, based on recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE) as enzyme and indoxyl acetate as substrate. The visible chromogenic strip had the advantages identical to those of commonly used lateral flow assays (LFAs) with utmost simplicity in sample loading and result observation. After optimization, depending on the color intensity (CI) values, the well-established assay has the capabilities of both qualitative measurement via naked eyes and quantitative analysis by colorimetric reader with the desirable IC_(50) values against the tested six insecticides (0.06 μgmL~(-1) of carbofuran, 0.28 μgm L~(-1) of methomyl, 0.03 μg mL~(-1) of dichlorvos, 31.6 μg mL~(-1) of methamidophos, 2.0 μg mL~(-1) of monocrotophos, 6.3 μLgmL~(-1) of omethoate). Acceptable matrix effects and satisfactory detection performance were confirmed by in-parallel LC-MS/MS analysis in different vegetable varieties at various spiked levels of 10~(-3) to 10~1 μg g~(-1). Overall, the testified suitability and applicability of this novel platform meet the requirements for practical use in food safety management and environmental monitoring, especially in the developing world.

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