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Production of triploid plants of papaya by endosperm culture

文献类型： 外文期刊

作者： Sun, De-Quan 1 ; Lu, Xin-Hua 1 ; Liang, Guo-Lu 2 ; Guo, Qi-Gao 2 ; Mo, Yi-Wei 1 ; Xie, Jiang-Hui 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, S Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China

2.Southwest Univ, Coll Hort & Landscape, Chongqing 400716, Peoples R China

关键词： Carica papaya;Endosperm culture;Callus induction;Regeneration;Triploids

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： Triploid papaya (Carica papaya) plants were obtained by immature endosperm culture. Visible callusing of the endosperm occurred 21 days after initiation of cultures. A continuously growing callus was observed and a maximum of 68.7% of callus induction frequency was obtained when immature endosperm with embryo was cultured on Murashige and Skoog (MS) medium containing 6.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 mu M alpha-naphthaleneacetic acid (NAA) and 4.0 mu M 6-furfurylamino purine (Kn). Shoot buds were produced when the callus was subcultured on a medium supplemented with 6-benzyladenine (BA) or thidiazuron (TDZ) along with NAA. Shoots were detached from the callus and transferred to the elongation medium supplemented with indole-3-acetic acid (IAA) and BA. The combination of 3.0 mu M IAA and 1.5 mu M BA was the best in terms of the number of cultures (93.8%) showing axillary shoot proliferation. The addition of 2.0 mu M indole-3-butyric acid (IBA) to the 1/2 MS medium was most effective at inducing root formation with 90% of the shoots developing four to five roots. Healthy rooted plantlets were transferred to pots containing sterilized bed soil and perlite (3:1) mixture in the greenhouse and 78% of the micropropagated plants survived transplantation. The leaves from endosperm-derived plants showed larger stomata and more chloroplasts in guard cells than that from the parent plants. Over 75% of the endosperm-derived plants were triploid with chromosome number 2n = 3x = 27.

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