您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Antioxidant effect of a phytoestrogen equol on cultured muscle cells of embryonic broilers

文献类型： 外文期刊

作者： Wei, Xiao-Jing 1 ; Wu, Jing 1 ; Ni, Ying-Dong 1 ; Lu, Li-Zhi 2 ; Zhao, Ru-Qian 1 ;

作者机构： 1.Nanjing Agr Univ, Key Lab Anim Physiol & Biochem, Nanjing 210095, Jiangsu, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Equol;Antioxidation;H2O2;Muscle cells;Broiler

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Previous studies have shown that the in ovo injection of equol can markedly improve the water-holding capacity of muscles of broilers chickens at 7 wk of age through promotion of the antioxidant status. We aimed to investigate directly the antioxidant effects of equol on muscle cells in broilers. Muscle cells were separated from leg muscle of embryos on the 11th day of incubation and treated with equol and H(2)O(2), either alone or together. Cells were pretreated with medium containing 1, 10, or 100 mu M equol for 1 h prior to the addition of 1 mM H(2)O(2) for a further 1 h. Photomicrographs of cells were obtained. Cell viability, malondialdehyde (MDA) content, and L-lactate dehydrogenase (LDH) activity in the cell supernatant, as well as intracellular total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities were determined. Treatment with 1 mM H(2)O(2) caused serious damage to cells, indicated by comets with no clear head region but a very apparent tail of DNA fragments. Pretreatment with low (1 mu M) but not high concentrations of equol (10 mu M) inhibited cell damage, while 100 mu M equol caused more serious damage than H(2)O(2) alone. Pretreatment with 1 mu M equol had no effect on cell viability, while pretreatment with 10 and 100 mu M equol significantly decreased cell viability in a dose-dependent manner. Compared with H(2)O(2) alone, pretreatment with low-dosage equol markedly decreased LDH activity and MDA production in the supernatant, significantly increased intracellular T-SOD activity (P < 0.05) and tended to increase intracellular GSH-Px activity (0.05 < P < 0.1). Pretreatment with high-dosage equol (10 and 100 mu M) significantly enhanced LDH activity, but had no effect on MDA content, T-SOD or GSH-Px activity induced by H(2)O(2,) except for an obvious increase in GSH-Px activity caused by 10 mu M equol. These results indicate that equol at low dosage can prevent skeletal muscle cell damage induced by H(2)O(2), while pretreatment with high-dosage equol shows a synergistic effect with H(2)O(2) in inducing cell damage.

  • 相关文献

[1]The effect of equol injection in ovo on posthatch growth, meat quality and antioxidation in broilers. Wei, X. J.,Ni, Y. D.,Zhao, R. Q.,Lu, L. Z.,Grossmann, R.. 2011

[2]The effect of equol injection in ovo on lipid metabolism and hepatic lipogenic gene expression in broilers. Ni, Y. D.,Wei, X. J.,Zhang, C. X.,Zhong, Y.,Zhao, R. Q.,Lu, L. Z.,Grossmann, R.. 2012

[3]Equol, a Clinically Important Metabolite, Inhibits the Development and Pathogenicity of Magnaporthe oryzae, the Causal Agent of Rice Blast Disease. Wang, Jiaoyu,Li, Ling,Yin, Yeshi,Gu, Zhuokan,Chai, Rongyao,Wang, Yanli,Sun, Guochang,Li, Ling.

[4]温度对油桃休眠花芽H_2O_2含量和呼吸代谢的影响. 徐文荣,闫田力,秦旭. 2008

[5]Effect of dietary daidzein supplementation on egg laying rate was associated with the change of hepatic VTG-II mRNA expression and higher antioxidant activities during the post-peak egg laying period of broiler breeders. Ni, Y. D.,Wu, J.,Tong, H. Y.,Huang, Y. B.,Zhao, R. Q.,Lu, L. Z.,Grossmann, R.. 2012

[6]Association of growth rate with hormone levels and myogenic gene expression profile in broilers. Xiao, Yingping,Li, Kaifeng,Gui, Guohong,Yang, Hua,Wu, Choufei,Zhang, Guolong. 2017

[7]Molecular analysis of intestinal bacterial microbiota of broiler chickens fed diets containing fermented cottonseed meal. Sun, H.,Fang, C. L.,Feng, J.,Tang, J. W.,Yao, X. H.,Wu, Y. F.,Wang, X.. 2013

[8]Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers. Sun, Hong,Feng, Jie,Tang, Jiang-wu,Yao, Xiao-hong,Wu, Yi-fei,Wang, Xin.

[9]Effects of dietary supplementation of synbiotics on growth performance, intestinal morphology, sIgA content and antioxidant capacities of broilers. Min, Y. N.,Yang, H. L.,Gao, Y. P.,Xu, Y. X..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台