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The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana

文献类型： 外文期刊

作者： Zhang, Chao 1 ; Pei, Xinwu 1 ; Wang, Zhixing 1 ; Jia, Shirong 1 ; Guo, Shiwei 2 ; Zhang, Yonggiang 1 ; Li, Weimin 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Crops, Jiangsu High Qual Rice R&D Ctr, Nanjing 210014, Jiangsu, Peoples R China

关键词： Cell-to-cell movement;Domain;Foliar necrosis;Long-distance movement;Pc4;RSV

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

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收录情况： SCI

摘要： The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K 122-D 258 of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR 122-124, D 135, ED 170-171, ER 201-202, EFE 218-220 or ELD 256-258 with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR 122-124 was linked with long-distance movement. The region D 17-K 33 was recognized as a crucial domain for leaf necrosis response, and mutagenesis of DD 17-18 or RK 32-33 greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana.

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