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Overexpression of Nelumbo nucifera metallothioneins 2a and 3 enhances seed germination vigor in Arabidopsis

文献类型： 外文期刊

作者： Zhou, Yuliang 1 ; Chu, Pu 1 ; Chen, Huhui 1 ; Li, Yin 1 ; Liu, Jun 2 ; Ding, Yu 3 ; Tsang, Edward W. T. 4 ; Jiang, Liwen; 1 ;

作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resource, Guangzhou 510275, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Guangzhou 510640, Peoples R China

3.Chinese Univ Hong Kong, Sch Life Sci, Ctr Cell & Dev Biol, Shatin, Hong Kong, Peoples R China

4.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

5.Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Taipei 106, Taiwan

关键词： Arabidopsis;Metallothionein;Oxidative stresses;Sacred lotus;Salt stress;Seed germination vigor

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

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收录情况： SCI

摘要： Metallothioneins (MTs) are small, cysteine-rich and metal-binding proteins which are involved in metal homeostasis and scavenging of reactive oxygen species. Although plant MTs have been intensively studied, their roles in seeds remain to be clearly established. Here, we report the isolation and characterization of NnMT2a, NnMT2b and NnMT3 from sacred lotus (Nelumbo nucifera Gaertn.) and their roles in seed germination vigor. The transcripts of NnMT2a, NnMT2b and NnMT3 were highly expressed in developing and germinating sacred lotus seeds, and were dramatically up-regulated in response to high salinity, oxidative stresses and heavy metals. Analysis of transformed Arabidopsis protoplasts showed that NnMT2a-YFP and NnMT3-YFP were localized in cytoplasm and nucleoplasm. Transgenic Arabidopsis seeds overexpressing NnMT2a and NnMT3 displayed improved resistance to accelerated aging (AA) treatment, indicating their significant roles in seed germination vigor. These transgenic seeds also exhibited higher superoxide dismutase activity compared to wild-type seeds after AA treatment. In addition, we showed that NnMT2a and NnMT3 conferred improved germination ability to NaCl and methyl viologen on transgenic Arabidopsis seeds. Taken together, these data demonstrate that overexpression of NnMT2a and NnMT3 in Arabidopsis significantly enhances seed germination vigor after AA treatment and under abiotic stresses.

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