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A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes

文献类型： 外文期刊

作者： Han, Zheng 2 ; Liu, Gang 2 ; Rao, Qinxiong 2 ; Bai, Bing 2 ; Zhao, Zhihui 2 ; Liu, Hong 1 ; Wu, Aibo 2 ;

作者机构： 1.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai 201403, Peoples R China

关键词： Liquid chromatography tandem mass spectrometry;Multiresidue analysis;Phytohormone

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of five acid/alkaline phytohormones, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthylacetic acid (NAA), gibberellic acid (GA _3) and isopentenyladenine (2IP), in grapes was developed. After optimization, the samples were extracted with methanol containing 1% formic acid and purified by Oasis HLB SPE cartridges. The analytes were separated on a Thermo Hypersil Gold column (100mm×2.1mm, 3.0μm) with water and acetonitrile, then determined with Thermo tandem quadrupole mass spectrometer operating in negative electro-spray ionization using selected reaction monitoring (SRM) mode. The established method was further validated by determining the linearity (R ~2≥0.9990), average recovery (82.5-105.4%), sensitivity (0.05-1.00ngmL ~(-1)), precision (RSD≤13.0%) and stability (RSD≥82.0%). Finally, the application of the approach proposed to thirty grape samples convinced its desirable performance for rapid analysis of multiclass phytohormones, supporting its sufficient capability for multiresidue analyses or other analytical system targeting phytohormones in agriculture field.

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