文献类型： 外文期刊

作者： Wang, L. M. ¹ ; Wen, J. X. ¹ ; Yuan, J. L. ¹ ; Cang, Ming ¹ ; Liu, D. J. ¹ ;

作者机构： 1.Inner Mongolia Univ, China Educ Minist Res Mammal Reprod Biol & Biotec, Key Lab, Hohhot 010021, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Shihezi 832000, Peoples R China

关键词： IGF;RNAi;In vitro;Bovine;Preimplantation embryo

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

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收录情况： SCI

摘要： This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1 (IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In oocytes injected with IGF-IR siRNA, the relative IGF-IR mRNA levels compared to controls were 28% and 46% at 6 and 24 h after injection, respectively. With respect to the injection of IGF-IR siRNA in zygotes, 24 h after injection the relative levels of IGF-IR mRNA and protein in the two-cell embryos were 74% and 78% of those in the controls, respectively. IGF-IR siRNA reduced blastocyst formation (23.2%) compared to siRNA controls (33.0%) and uninjected oocytes (35.4%; P〈0.05) and the number of viable cells per IGF-IR siRNA-treated blastocyst (64 ± 3) was significantly reduced, compared to control siRNA and uninjected blastocysts (81 ± 3 and 116 ± 4; P〈0.01). In conclusion, IGF-IR siRNA knockdown reduces the development of bovine embryos, and microinjection in zygotes can decrease blastocyst cell number.