您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango (Mangifera indica L.) cotyledon segments

文献类型： 外文期刊

作者： Li, Yun-He 1 ; Zou, Ming-Hong 1 ; Feng, Bi-Hong 2 ; Huang, Xia 3 ; Zhang, Zhi 1 ; Sun, Guang-Ming 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, S Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

3.Sun Yat Sen Univ, Key Lab Gene Engn, Minist Educ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

关键词： Adventitious root;Auxin efflux carrier;Auxin influx carrier;Cotyledon segment;Mangifera indica L.;Polar auxin transport

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Polar auxin transport (PAT) plays an important role in the adventitious root formation of mango cotyledon segments, but the molecular mechanism remains unclear. In this study, we cloned a gene encoding an auxin efflux carrier (designated as MiPIN1), and we cloned four genes encoding auxin influx carriers (designated as MiAUX1, MiAUX2, MiAUX3 and MiAUX4). The results of a phylogenetic tree analysis indicated that MiPIN1 and the MiAUXs belong to plant PIN and AUXs/LAXs groups. Quantitative real-time PCR indicated that the expression of MiPIN1 and the MiAUXs was lowest at 0 days but sharply increased on and after day 4. During the root formation in the mango cotyledon segments, the MiPIN1 expression in the distal cut surface (DCS) was always higher than the expression in the proximal cut surface (PCS) whereas the expression of the MiAUXs in the PCS was usually higher than in the DCS. This expression pattern might be result in the PAT from the DCS to the PCS, which is essential for the adventitious root formation in the PCS. Our previous study indicated that a pre-treatment of embryos with indole-3-butyric acid (IBA) significantly promoted adventitious rooting in PCS whereas a pre-treatment with 2,3,5-triiodobenzoic acid (TIBA) completely inhibited this rooting. In this study, however, IBA and TIBA pre-treatments slightly changed the expression of MiPIN1. In contrast, while the MiAUX3 and MiAUX4 expression levels were significantly up-regulated by the IBA pre-treatment, the expression levels were down-regulated by the TIBA pre-treatment. These findings imply that MiAUX3 and MiAUX4 are more sensitive to the IBA and TIBA treatments and that they might play important roles during adventitious root formation in mango cotyledon segments.

  • 相关文献

[1]Construction of a High-Density Genetic Map Based on Large-Scale Marker Development in Mango Using Specific-Locus Amplified Fragment Sequencing (SLAF-seq). Luo, Chun,Shu, Bo,Yao, Quangsheng,Wu, Hongxia,Xu, Wentian,Wang, Songbiao. 2016

[2]Resistance of Botryodiplodia theobromae to Carbendazim and the Fungicides Screening for Mango Stem End Rot Control. Li, M.,Gao, Z. Y.,Zhang, Z. K.,Yang, F. Z.,Li, M.,Gao, Z. Y.,Zhang, Z. K.,Yang, F. Z.,Li, M..

[3]Effect of Bagging on the Composition of Carbohydrate, Organic Acid and Carotenoid Contents in Mango Fruit. Zhao, Jia-Ju,Wang, Jia-Bao,Zhang, Xin-Chun,Li, Huan-Ling,Gao, Zhao-Yin.

[4]Transcriptional sequencing and analysis of major genes involved in the adventitious root formation of mango cotyledon segments. Li, Yun-He,Zhang, Hong-Na,Wu, Qing-Song,Li, Yun-He,Li, Yun-He,Muday, Gloria K..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台