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Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China

文献类型： 外文期刊

作者： Wang, Jiansheng 1 ; Gu, Honghui 1 ; Yu, Huifang 1 ; Zhao, Zhenqing 1 ; Sheng, Xiaoguang 1 ; Zhang, Xiaohui 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China

关键词： broccoli;glucosinolate;sulforaphane;anti-cancer

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： Five main cultivars from China and 143 parent materials grown in a greenhouse were used to investigate the glucosinolates in broccoli florets. Eight aliphatic glucosinolates, four indole glucosinolates, and one aromatic glucosinolate were identified and quantified using high-performance liquid chromatography (HPLC). The results showed that glucoraphanin, glucobrassicin, 4- methoxyglucobrassicin and neoglucobrassicin were present in all samples. However, the predominant type of glucosinolate was different among pure lines. The anti- cancer glucoraphanin concentration ranged from 0.06 to 24.17 μmol/g in pure lines and from 1.57 to 5.95 μmol/g in commercial cultivars. The progoitrin concentration in commercial cultivars varied from 1.77 to 6.07 μmol/g with a mean value of 3.20 μmol/g. Significant variations were observed in the concentration of individual glucosinolates and in each class of glucosinolates among broccoli populations. Dozens of specific lines with altered glucosinolate profiles, as well as ten good candidates for breeding high-chemoprotective glucosinolate cultivars, were obtained according to the putative glucosinolate pathway in broccoli.

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[2]Development and Validation of High-glucoraphanin Broccoli F-1 Hybrids and Parental Lines. Gu, Honghui,Wang, Jiansheng,Yu, Huifang,Zhao, Zhenqing,Sheng, Xiaoguang,Chen, Jisuan,Xu, Yingjun. 2014

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[4]Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance. Jiang Ming,He Cai-ming,Miao Li-xiang,Zhang Yu-chao. 2012

[5]An efficient shoot regeneration system and Agrobacterium-mediated transformation with codA gene in a doubled haploid line of broccoli. Sheng, Xiaoguang,Gu, Honghui,Yu, Huifang,Wang, Jiansheng,Zhao, Zhenqing,Qi, Zirong. 2016

[6]HEAVY METAL CONTENT IN BR CCOLI (BRASSICA OLERACEA L. VAR. ITALICA PLENCK) AND POTENTIAL HEALTH RISK IN ZHEJIANG PROVINCE, CHINA. Sun, Caixia,Zhang, Qi,Xiao, Wendan,Xu, Ping,Xiang, Shuo. 2017

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