A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization
文献类型： 外文期刊
作者： Wang, Ziyuan 1 ; Jin, Yi 1 ; Wu, Huijun 1 ; Tian, Zhaofeng 2 ; Wu, Yuying 3 ; Xie, Xiangming 1 ;
作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
3.Beijing Forestry Univ, Coll Mat Sci & Technol, Beijing 100083, Peoples R China
关键词： Saccharomonospora viridis;Alkali-tolerant;Thermostability;Xylanase
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）
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收录情况： SCI
摘要： A xylanase gene, designated Svixyn10A, was cloned from actinomycetes Saccharomonospora viridis and the gene product was characterized. Gene Svixyn10A contains 1,374 bp and encodes a polypeptide of 457 amino acids composed of a glycoside hydrolase family 10 catalytic domain with a putative signal peptide, a short Gly-rich linker and a family 2 carbohydrate-binding module (CBM). The deduced amino acid sequence of SviXyn10A shared the highest identity (57 %) with a hypothetical xylanase from Streptomyces lividans TK24 (ZP_05528201). A recombinant His-tagged xylanase, SviXyn10A was expressed in Escherichia coli BL21 and purified. The optimum pH and temperature for SviXyn10A is 8.0 and 60 A degrees C. Compared with thermophilic and mesophilic counterparts, SviXyn10A was more active at high temperatures, retaining > 63 % of its maximum activity at 65-70 A degrees C and similar to 40 % even at 80 A degrees C. It had broad pH adaptability (> 35 % activity at pH 5.0-11.0) and alkali-tolerance (> 70 % activity after incubation at pH 8.0-11.0 for 1 h at 37 A degrees C), and was highly thermostable (> 75 % activity after incubation at 70 A degrees C for 3 h at pH 8.0). It may be the first alkali-tolerant thermostable xylanase reported from Saccharomonospora. These favorable properties make SviXyn10A a good candidate for application in pulp and paper industries.
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