Identification and characterization of genes related to the development of breast muscles in Pekin duck
文献类型: 外文期刊
作者: Xu, Tieshan 1 ; Huang, Wei 1 ; Zhang, Xiaohui 3 ; Ye, Baoguo 4 ; Zhou, Hanlin 2 ; Hou, Shuisheng 1 ;
作者机构: 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Res Inst, Danzhou 571737, Hainan, Peoples R China
3.Henan Univ Sci & Technol, Coll Anim Sci, Luoyang 471003, Henan, Peoples R China
4.Hainan Acad Agr Sci, Inst Anim Sci & Vet, Haikou 571100, Peoples R China
关键词: Pekin duck;Skeletal muscle;Suppression subtractive hybridization;Gene expression
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Pekin Duck is world-famous for its fast growth, but its breast muscle development is later and breast muscle content is lower compared with other muscular ducks. Therefore, it is very important to discover the genetic mechanism between breast muscle development and relative gene expression in Pekin duck. In current study, the genes which have relationships with breast muscle development were identified by suppression subtractive hybridization. A total of 403 positive clones were sequenced and 257 unigenes were obtained. The expression of 23 genes were analyzed in the breast muscle of 2-, 4-, 6-, 8- week old Pekin ducks. The results showed that unknown clone A233, C83 and C99 showed descending tendency as age increased; KBTBD10, HSPA8, MYL1, ZFP622, MARCH4, Nexilin, FABP4 and MUSTN1 had high expression levels at 6 weeks old; WAC, NT5C3, HSP90AA1, MRPL33, KLF6, TSNAX, CDC42EP3, HSPA4, TRAK1, NR2F2, HAUS1 and IGF1 had high expression levels at 8 weeks and showed ascending tendency as age increased. Expression of these 23 genes were also analyzed in breast muscle, leg muscle, heart, kidney, liver, muscular stomach and sebum cutaneum in 4-8-week old Pekin duck and results showed that most of these genes had high expression in breast muscle, leg muscle and heart.
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