Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation
文献类型： 外文期刊
作者： Hong, Ni 2 ; Chen, Songlin 3 ; Ge, Ruowen 2 ; Song, Jianxing 2 ; Yi, Meisheng 1 ; Hong, Yunhan 2 ;
作者机构： 1.Sun Yat Sen Univ, Sch Marine Sci, Guangzhou 510275, Guangdong, Peoples R China
2.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
3.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
期刊名称：STEM CELLS AND DEVELOPMENT （ 影响因子：3.272； 五年影响因子：3.885 ）
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收录情况： SCI
摘要： Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species. Parameters influencing interspecies chimera formation have remained poorly elucidated. Here, we report interordinal chimera formation between medaka and zebrafish, which separated ～320 million years ago and exhibit a more than 2-fold difference in developmental speed. We show that, on transplantation into zebrafish blastulae, both noncultivated blastomeres and long-term cultivated embryonic stem (ES) cells of medaka adopted the zebrafish developmental program and differentiated into physiologically functional cell types including pigment cells, blood cells, and cardiomyocytes. We also show that medaka ES cells express differentiation gene markers during chimeric embryogenesis. Therefore, the evolutionary distance and different embryogenesis speeds do not produce donor-host incompatibility to compromise chimera formation between medaka and zebrafish, and molecular markers are valuable for analyzing lineage commitment and cell differentiation in interspecific chimeric embryos.
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