Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation
文献类型： 外文期刊
作者： Hong, Ni 2 ; Chen, Songlin 3 ; Ge, Ruowen 2 ; Song, Jianxing 2 ; Yi, Meisheng 1 ; Hong, Yunhan 2 ;
作者机构： 1.Sun Yat Sen Univ, Sch Marine Sci, Guangzhou 510275, Guangdong, Peoples R China
2.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
3.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
期刊名称：STEM CELLS AND DEVELOPMENT （ 影响因子：3.272； 五年影响因子：3.885 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species. Parameters influencing interspecies chimera formation have remained poorly elucidated. Here, we report interordinal chimera formation between medaka and zebrafish, which separated ～320 million years ago and exhibit a more than 2-fold difference in developmental speed. We show that, on transplantation into zebrafish blastulae, both noncultivated blastomeres and long-term cultivated embryonic stem (ES) cells of medaka adopted the zebrafish developmental program and differentiated into physiologically functional cell types including pigment cells, blood cells, and cardiomyocytes. We also show that medaka ES cells express differentiation gene markers during chimeric embryogenesis. Therefore, the evolutionary distance and different embryogenesis speeds do not produce donor-host incompatibility to compromise chimera formation between medaka and zebrafish, and molecular markers are valuable for analyzing lineage commitment and cell differentiation in interspecific chimeric embryos.
- 相关文献
作者其他论文 更多>>
-
Decoding the fish genome opens a new era in important trait research and molecular breeding in China
作者：Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Li, Jiongtang
关键词：fish genomics; economic traits; genomic selection; genome editing; genomic breeding
-
Integrative omics reveals clues as to why Cynoglossus semilaevis pseudomales produce no W sperm
作者：Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Wang, Liping;Xu, Feng;Ye, Zhan;Chen, Songlin;Chen, Songlin;Chen, Songlin;Li, Hengde
关键词：Spermatogenesis; Transcriptome; Proteome; Ubiquitination; Pseudomale; Cynoglossus semilaevis
-
Multi-organ transcriptomic profiles and gene-regulation network underlying vibriosis resistance in tongue sole
作者：Chen, Quanchao;Hu, Guobin;Ma, Xinran;Wang, Jie;Shi, Meng;Chen, Songlin;Zhou, Qian;Chen, Songlin;Zhou, Qian
关键词：
-
Transcriptome Analysis Reveals the Immunosuppression in Tiger Pufferfish (Takifugu rubripes) under Cryptocaryon irritans Infection
作者：Chi, Yong;Chen, Songlin;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Zhang, Hongxiang;Zhang, Haien;Li, Weidong;Li, Yangzhen;Zhang, Hongxiang;Robledo, Diego
关键词：fugu; RNA-seq; immune response; parasite; aquaculture
-
Characterization of emopamil binding protein (Cs-ebp) reveals its potential role in cholesterol biosynthesis and growth performance of Chinese tongue sole
作者：Chen, Zhangfan;Li, Xihong;Wang, Na;Chen, Songlin;Chen, Zhangfan;Li, Xihong;Cheng, Peng;Mai, Jiaqi;Sun, Yuqi;Wang, Jiacheng;Shi, Rui;Zhang, Qi;Wang, Na;Chen, Songlin;Chen, Zhangfan;Li, Xihong;Cheng, Peng;Mai, Jiaqi;Sun, Yuqi;Wang, Jiacheng;Shi, Rui;Zhang, Qi;Wang, Na;Chen, Songlin;Wang, Na;Chen, Songlin
关键词：Chinese tongue sole; Emopamil-binding protein (EBP); Cholesterol biosynthesis; Sexual size dimorphism; Ovarian development
-
Development of a 20 K SNP array for the leopard coral grouper, Plectropomus leopardus
作者：Zhou, Qian;Lu, Sheng;Liu, Yang;Chen, Songlin;Zhou, Qian;Lu, Sheng;Liu, Yang;Chen, Songlin;Zhou, Bo;Zhou, Qian;Chen, Songlin
关键词：Leopard coral grouper; Plectropomus leopardus; SNP array; Aquaculture
-
Accurate genomic selection using low-density SNP panels preselected by maximum likelihood estimation
作者：Qu, Shiyu;Lu, Sheng;Liu, Yang;Li, Ming;Chen, Songlin;Qu, Shiyu;Lu, Sheng;Liu, Yang;Li, Ming;Chen, Songlin;Qu, Shiyu;Lu, Sheng;Liu, Yang;Li, Ming;Chen, Songlin;Chen, Songlin
关键词：Maximum likelihood estimation; Genomic selection; SNP pre-selection; MLE-rank
