Vacuum infiltration transformation of non-heading Chinese cabbage (Brassica rapa L. ssp chinensis) with the pinII gene and bioassay for diamondback moth resistance
文献类型： 外文期刊
作者： Zhang, Junjie 1 ; Liu, Fan 1 ; Yao, Lei 4 ; Luo, Chen 5 ; Zhao, Qing 4 ; Huang, Yubi 3 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China
3.Sichuan Agr Univ, Coll Agron, Yaan 625014, Sichuan, Peoples R China
4.Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China
5.Beijing Acad Agr & Forestry Sci, Plant Protect & Environm Protect Inst, Beijing 100097, Peoples R China
关键词： Non-heading Chinese cabbage;Vacuum infiltration transformation;pinII gene;Insect resistance
期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）
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收录情况： SCI
摘要： Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is a popular vegetable in Asian countries. The diamondback moth (DBM), Plutella xylostella (L.), an insect with worldwide distribution, is a main pest of Brassicaceae crops and causes enormous crop losses. Transfer of the anti-insect gene into the plant genome by transgenic technology and subsequent breeding of insect-resistant varieties will be an effective approach to reducing the damage caused by this pest. We have produced transgenic non-heading Chinese cabbage plants expressing the potato proteinase inhibitor II gene (pinII) and tested the pest resistance of these transgenic plants. Non-heading Chinese cabbages grown for 45 days on which buds had formed were used as experimental materials for Agrobacterium-mediated vacuum infiltration transformation. Forty-one resistant plants were selected from 1166 g of seed harvested from the infiltrated plants based on the resistance of the young seedlings to the herbicide Basta. The transgenic traits were further confirmed by the Chlorophenol red test, PCR, and genomic Southern blotting. The results showed that the bar and pinII genes were co-integrated into the resistant plant genome. A bioassay of insect resistance in the second generation of individual lines of the transgenic plants showed that DBM larvae fed on transgenic leaves were severely stunted and had a higher mortality than those fed on the wild-type leaves.
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