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Simple colorimetric DNA detection based on hairpin assembly reaction and target-catalytic circuits for signal amplification

文献类型： 外文期刊

作者： Ma, Cuiping 1 ; Wang, Wenshuo 2 ; Li, Zhaoxin 1 ; Cao, Lijie 2 ; Wang, Qingyin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Shandong Prov Key Lab Biochem Anal, Qingdao 266042, Peoples R China

关键词： Biosensors;Colorimetric detection;Hairpin assembly;Nanostructures;Signal amplification

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

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收录情况： SCI

摘要： A simple strategy of colorimetric DNA detection is presented based on a hairpin assembly reaction and target-catalytic DNA circuits to achieve enzyme-free signal amplification. The method employed two hairpin species (H1 and H2), which were stable and unable to hybridize in the absence of target. In the presence of target, the target hybridized with hairpin H1 and the opened hairpin H1 hybridized with hairpin H2, allowing the target to be displaced. H1 and H2 were respectively attached to gold nanoparticles, allowing the duplex formed from H1 and H2 to be visualized with the naked eye. The displaced target again triggered the next round of strand exchange reaction to achieve signal amplification. The method may have a wide range of sensor applications because it is enzyme-free and simple to perform.

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