Analysis of genetic diversity and population structure of Pleuronectes yokohamae indicated by AFLP markers
文献类型: 外文期刊
作者: Zhang, Hui 1 ; Yu, Han 1 ; Gao, Tianxiang 2 ; Zhang, Yan 1 ; Han, Zhiqiang 3 ; Xiao, Yongshuang 4 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
3.Zhejiang Ocean Univ, Fishery Coll, Zhoushan 316004, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
关键词: Pleuronectes yokohamae;AFLP;Genetic diversity;Population structure
期刊名称:BIOCHEMICAL SYSTEMATICS AND ECOLOGY ( 影响因子:1.381; 五年影响因子:1.351 )
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收录情况: SCI
摘要: Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity and variation of two populations of Pleuronectes yokohamae from China and North Korea. A total of 228 loci ranging in size were detected from 63 individuals, of which 139 were polymorphic. The number of bands per primer combination varied from 37 to 51 and the percentage of polymorphic bands per primer combination ranged from 43.14% to 72.97%.The proportion of polymorphic loci, the Nei's genetic diversity and Shannon genetic diversity index of China and North Korea populations were 55.09% and 55.71%, 0.1205 and 0.1917, 0.1370 and 0.2129, respectively. The results showed that the genetic diversity of these two populations was at the same level. Dominant gene frequency revealed that these two populations have the same genetic population structure. F-st value of the two populations was 0.0986 and AMOVA analysis indicated that 80.75% genetic variation came from individuals within populations, and there were also 19.25% genetic differentiation occurred between the two populations. There was significant genetic difference between two populations. Exact-p test showed there was no random genetic communication between two populations. The UPGMA and NJ trees based on AFLP data supported this result
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