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Rapid estimation of lipid content in an Antarctic ice alga (Chlamydomonas sp.) using the lipophilic fluorescent dye BODIPY505/515

文献类型： 外文期刊

作者： Mou, Shanli 1 ; Xu, Dong 2 ; Ye, Naihao 2 ; Zhang, Xiaowen 2 ; Liang, Chengwei 3 ; Liang, Qiang 1 ; Zheng, Zhou 1 ; Zhuan 1 ;

作者机构： 1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China

关键词： Chlamydomonas sp. ICE-L;Lipid content;BODIPY505/515;Rapid estimation

期刊名称：JOURNAL OF APPLIED PHYCOLOGY （ 影响因子：3.215； 五年影响因子：3.612 ）

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收录情况： SCI

摘要： Chlamydomonas sp. ICE-L, isolated from Antarctic coastal marine environments, was selected as a high lipid producer, which may be useful for biodiesel production. The lipophilic fluorescent dye BODIPY505/515 was used to determine the algal lipid content. Lipid bodies stained with BODIPY505/515 have a characteristic green fluorescence, and their volumes were determined using the sphere volume formula. In this study, lipid accumulation by Chlamydomonas ICE-L was analyzed under different cultivation conditions (nitrogen deficiency and UV-B radiation). The results demonstrated that nitrogen deficiency and UV-B radiation could significantly promote the accumulation of lipid content per cell. The highest yields of total lipid content (reaching 84 μL L1) were obtained in full Provasoli medium after 12 days of cultivation, but not in the nitrogen-deficient medium. The inoculum used in this experiment was obtained from the late-exponential growth phase. The main reason was that the cell numbers in nitrogen-deficient medium had not increased and total lipid contents were offset by the lower growth rate. Considering the high lipid content in Chlamydomonas sp. ICE-L, this alga might be a promising alternative species for production of microalgal oil for the production of renewable biodiesel in the future.

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