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Molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from Simensis crocodile (Crocodylus siamensis)

文献类型： 外文期刊

作者： Hao, Juan 1 ; Li, Yan-Wei 1 ; Xie, Ming-Quan 1 ; Li, An-Xing 1 ;

作者机构： 1.Sun Yat Sen Univ, Key Lab Aquat Prod Safety, Minist Educ, Sch Life Sci,State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Agrobiotech Res Inst, Guangzhou 510610, Guangdong, Peoples R China

关键词： Antibacterial activity;Crocodylus siamensis;Hepcidin

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

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收录情况： SCI

摘要： Hepcidin, a cysteine-rich cationic antibacterial peptide, plays an important role in human defense against pathogen infection. However, its role in reptile immune response and whether it is involved in antibacterial immune have not yet been proven. In order to study the antibacterial activity of Crocodylus siamensis hepcidin (Cshepc), a common reptile which lives in topic region of Southeast Asia, a cDNA sequence of Cshepc was cloned, which included an open reading frame (ORF) of 300. bp encoding a 99 amino acid preprohepcidin. Cshepc has eight cysteines formed four conserved disulfide bridges, similarly to that of human's. Sequence analysis showed that Cshepc mature peptide was more conserved than that of preprohepcidin. Tissue expression analysis indicated that Cshepc transcripts were highly expressed in the liver, muscle and heart of C. siamensis. Recombinant expressed hepcidin could significantly inhibit the growth of the Gram-negative bacteria Escherichia coli and Aeromonas sobria as well as the Gram-positive bacterium Staphylococcus aureus, and Bacillus subtilis in vitro, suggesting that Cshepc, like human hepcidin could play a role in the antibacterial function in hosts innate immune response.

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