文献类型： 外文期刊

作者： Wang, Chuan Tang ¹ ; Yu, Hong Tao ¹ ; Wang, Xiu Zhen ¹ ; Tang, Yue Yi ¹ ; Wu, Qi ¹ ; Gao, Hua Yuan ² ; Hu, Dong Qing ³ ;

作者机构： 1.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China

2.Jilin Acad Agr Sci, Inst Econ Plants, Gongzhuling 136105, Peoples R China

3.Qingdao Entry Exit Inspect & Quarantine Bur, Qingdao 266002, Peoples R China

4.Quanzhou Agr Res Inst, Quanzhou 362212, Peoples R China

关键词： Hormone treatment;In vitro technique;Intersectional hybrid;Peanut;Seed development;Wild species

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

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收录情况： SCI

摘要： To broaden the narrow gene base of the cultivated peanut and utilize sources of resistance to abiotic/biotic stresses, quality-related traits and high yielding factors, much attention has been paid to wild relatives of the oilseed crop since 1980s. Species outside section Arachis are cross-incompatible with A. hypogaea L.; even some of the species from section Arachis, which are supposed to be compatible, may also encounter obstacles when crossed with the peanut cultigen. The objective of the present communication is to study the effects of simple hormone treatment on production of true incompatible hybrids in peanut to replace the tedious and lengthy in vitro embryo rescue procedures currently in use. In the incompatible cross, A. hypogaea cv Qunyu 101 × A. paraguariensis, post-pollination application of a hormone aqueous solution (IAA 4 mg/L + GA 2 mg/L) at flower bases resulted in 42 seeds, of which 26 were identified as true hybrids by allele-specific PCR for FAD2A genotyping. As a desirable alternative to in vitro embryo/ovules/peg culture, the present method may facilitate the utilization of wild species in peanut breeding. The method described here for peanut may be of reference to other crop plants where embryo abortion is also a problem.