文献类型： 外文期刊

作者： Tang, Yue Yi ² ; Wang, Chuan Tang ¹ ; Yang, Guan Pin ² ; Feng, Tong ³ ; Gao, Hua Yuan ³ ; Wang, Xiu Zhen ¹ ; Chi, Xiao ¹ ;

作者机构： 1.Shandong Peanut Res Inst, Qingdao, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China

3.Jilin Acad Agr Sci, Inst Econ Plants, Gongzhuling, Peoples R China

4.China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst CNTC, Zhengzhou, Peoples R China

关键词： chilling response;peanut;real-time quantitative PCR;suppression subtractive hybridization

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2011 年 14 卷 5 期

页码：

收录情况： SCI

摘要： To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2 degrees C and 15 degrees C, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).