您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Retrotransposon-microsatellite amplified polymorphism, an electrophoretic approach for studying genetic variability among Schistosoma japonicum geographical isolates

文献类型： 外文期刊

作者： Li, Juan 1 ; Zhao, Guang-Hui 1 ; Zhou, Dong-Hui 1 ; Sugiyama, Hiromu 5 ; Nisbet, Alasdair J. 1 ; Li, Xiao-Yan 3 ; Zou, 1 ;

作者机构： 1.CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China

2.S China Agr Univ, Guangzhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou, Guangdong, Peoples R China

4.NW A&F Univ, Yangling, Shaanxi, Peoples R China

5.Natl Inst Infect Dis, Dept Parasitol, Tokyo, Japan

6.Yunnan Agr Univ, Coll Anim Sci & Technol, Yunnan, Peoples R China

7.Dali Univ, Sch Basic Med, Dali, Yunnan, Peoples R China

8.Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai, Peoples R China

关键词： China;Genetic diversity;Retrotransposon-microsatellite amplified polymorphism (REMAP);Retrotransposons;Schistosoma japonicum

期刊名称：ELECTROPHORESIS （ 影响因子：3.535； 五年影响因子：2.731 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, retrotransposon-microsatellite amplified polymorphism (REMAP) was used to examine genetic variability among Schistosoma japonicum isolates from different endemic provinces in mainland China, using S. japonicum from Japan and the Philippines for comparison. Of the 50 primer combinations screened, eight produced highly reproducible REMAP fragments. Using these primers, 190 distinct DNA fragments were generated in total, of which 147 (77.37%) were polymorphic, indicating considerable genetic variation among the 43 S. japonicum isolates examined. The percentage of polymorphic bands (PPB) among S. japonicum isolates from mainland China, Japan, and the Philippines was 77.37%; PPB values of 18.42% and 53.68% were found among isolates from southwestern (SW) China and the lower Yangtze/Zhejiang province in eastern (E) China, respectively. Based on REMAP profiles, unweighted pair-group method with arithmetic averages (UPGMA) dendrogram analysis revealed that all of the S. japonicum samples grouped into three distinct clusters: parasites from mainland China, Japan, and the Philippines were clustered in each individual clade. Within the mainland China cluster, SW China isolates (from Sichuan and Yunnan provinces) grouped together, whereas worms from E China (Zhejiang, Anhui, Jiangxi, Jiangsu, Hunan, and Hubei provinces) grouped together. These results demonstrated that the REMAP marker system provides a reliable electrophoretic technique for studying genetic diversity and population structures of S. japonicum isolates from mainland China, and could be applied to other pathogens of human and animal health significance. ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  • 相关文献

[1]Genetic variability among Schistosoma japonicum isolates from the Philippines, Japan and China revealed by sequence analysis of three mitochondrial genes. Chen, Fen,Li, Juan,Zhou, Dong-Hui,Song, Hui-Qun,Zhu, Xing-Quan,Chen, Fen,Li, Juan,Sugiyama, Hiromu,Zhao, Guang-Hui,Zhu, Xing-Quan.

[2]Genome composition and genetic diversity of Musa germplasm from China revealed by PCR-RFLP and SSR markers. Ning, Shu-Ping,Xu, Lin-Bing,Lu, Yan,Huang, Bing-Zhi,Ge, Xue-Jun. 2007

[3]Analysis of the Germplasm Resources and Genetic Relationships among Cymbidium tortisepalum Cultivars Using Amplified Fragment Length Polymorphism Markers. X.Y.Wu,D.M.Li,G.F.Zhu. 2013

[4]A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Li, Juan,Li, Juan,Zhu, Xing-Quan,Chen, Fen,Sugiyama, Hiromu,Blair, David,Lin, Rui-Qing.

[5]The dopamine D2 receptor gene polymorphisms associated with chicken broodiness. Xu, H. P.,Shen, X.,Zhou, M.,Luo, C. L.,Kang, L.,Liang, Y.,Zeng, H.,Nie, Q. H.,Zhang, D. X.,Zhang, X. Q.,Zhou, M.,Kang, L.. 2010

[6]RAPD analysis of 33 varieties of banana. Su, J,Zhou, P,Zheng, XQ,Huang, BZ,Li, FN. 2001

[7]DNA fingerprinting of kiwifruit (Actinidia spp.) genotypes using fluorescent amplified fragment length polymorphism. Xu, X. B.,Chen, H.,Yi, G. J.,Zhang, Q. M.. 2007

[8]Development and evaluation of multi-genotype varieties of rice derived from MAGIC lines. Li, Xiao-Fang,Liu, Zhi-Xia,Lu, Dong-Bo,Liu, Yan-Zhuo,Mao, Xing-Xue,Li, Xiao-Fang,Li, Zhi-Xin,Li, Xiao-Fang,Li, Hua-Jun. 2013

[9]Developing a core collection of litchi (Litchi chinensis Sonn.) based on EST-SSR genotype data and agronomic traits. Sun, Qingming,Bai, Lijun,Xiang, Xu,Zhao, Junsheng,Ou, Liangxi,Ke, Lixiang. 2012

[10]Analysis of the Germplasm Resources and Genetic Relationships among Cymbidium tortisepalum Cultivars Using Amplified Fragment Length Polymorphism Markers. Wu, X. Y.,Li, D. M.,Zhu, G. F.. 2013

[11]Multilocus sequence typing of Enterocytozoon bieneusi in nonhuman primates in China. Karim, Md Robiul,Wang, Rongjun,Zhang, Longxian,Dong, Haiju,Qi, Meng,Jian, Fuchun,Zhang, Sumei,Ning, Changshen,He, Xiaoyi,Li, Jian,Rume, Farzana Islam,Sun, Mingfei,Yang, Guangyou,Zoug, Fengcai,Xiao, Lihua.

[12]Genetic diversity within Oryza rufipogon germplasms preserved in Chinese field gene banks of wild rice as revealed by microsatellite markers. Zhang, Chi-Hong,Li, Dao-Yuan,Pan, Da-Jian,Jia, Ji-Zeng,Dong, Yu-Shen.

[13]High prevalence of torque teno sus virus in China and genetic diversity of the 5' non-coding region. Long, Jin-Xue,Gao, Fei,Yuan, Shi-Shan,Tong, Guang-Zhi,Wei, Zu-Zhang,Zhai, Shao-Lun,Wei, Wen-Kang,Chen, Qin-Ling,Lv, Dian-Hong,Wu, Da-Cheng,Zhai, Shao-Lun,Luo, Man-Lin.

[14]Core EST-SSR Marker Selection Based on Genetic Linkage Map Construction and Their Application in Genetic Diversity Analysis of Litchi (Litchi chinensis Sonn.) Germplasm Resources. Xiang, X.,Chen, D. M.,Ma, S. P.,Ma, W. C.,Fan, J.,Yang, X. Y.,Liu, M.,Sun, Q. M.. 2014

[15]SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) FOR STUDYING GENETIC DIVERSITY AND POPULATION STRUCTURE OF PLANTS AND OTHER LIVING ORGANISMS: A PROTOCOL. Li, X. Y.,Zhao, J.,Yang, F.,Fu, Q. W.,Liu, H. S.,Wang, D. D.,Yang, Y. C.,Li, J.,Wang, R. Y.. 2014

[16]The Advancement of Research on Banana Germplasm Resources in China. Wu, Y. L.,Yi, G. J.,Huang, B. Z.,Wei, Y. R.,Li, C. Y.,Hu, C. H.,Huang, Y. H.. 2013

[17]Large-scale development of EST-SSR markers in sponge gourd via transcriptome sequencing. Wu, Hai-Bin,Gong, Hao,Liu, Peng,He, Xiao-Li,Luo, Shao-Bo,Zheng, Xiao-Ming,Zhang, Chang-Yuan,He, Xiao-Ming,Luo, Jianning.

[18]Biological characterization and genetic diversity analysis of two species of Pestalotiopsis causing twig dieback of Myrica rubra. Chen, Fangyong,Lu, Lianming,Wang, Yin,Ni, Haizhi,Wang, Dongmi,Du, Zhenguo.

[19]Development, characterization, and variability analysis of microsatellites from a commercial cultivar of Musa acuminata. Wu, Y. T.,Wang, J. Y.,Zheng, L. S.,Liu, W. L.,Huang, B. Z..

[20]Genetic diversity of Sporisorium scitamineum in mainland China assessed by SCoT analysis. Shen, Wan-kuan,Xu, Gang-hong,Luo, Ming-zhu,Jiang, Zi-de.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台