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Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck

文献类型: 外文期刊

作者: He, Jun 1 ; Tian, Yong 1 ; Li, Jinjun 1 ; Shen, Junda 1 ; Tao, Zhengrong 1 ; Fu, Yan 2 ; Niu, Dong 2 ; Lu, Lizhi 1 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China

关键词: Duck;L-FABP;SiRNA;Fat metabolism-related gene;Oleic acid

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Liver fatty acid binding protein (L-FABP) is a member of intracellular lipid-binding proteins responsible for the transportation of fatty acids. The expression pattern of duck L-FABP mRNA was examined in this study by quantitative RT-PCR. The resultsshowed that duck L-FABP gene was expressed in many tissues, including heart, lung, kidney, muscle, ovary, brain, intestine, stomach and adipocyte tissues, and highly expressed in liver. Several lipid metabolism-related genes were selected to detect the regulation of L-FABP in duck. The expression of L-FABP and lipoprotein lipase was promoted by oleic acid. The L-FABP knockdown decreased the expression levels of peroxisome proliferator-activated receptor alpha (PPAR alpha), fatty acid synthase and lipoprotein lipase by 61.1, 42.3 and 53.7 %, respectively (P < 0.05), but had no influences on the mRNA levels of PPAR gamma and leptin receptor. L-FABP might function through the PPAR alpha to regulate the fat metabolism-related gene expression and play important roles in lipid metabolism in duck hepatocytes.

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