您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and molecular characterization of a C-type lectin-like protein from Chinese shrimp (Fenneropenaeus chinensis)

文献类型： 外文期刊

作者： Lai, Xiaofang 1 ; Kong, Jie 2 ; Wang, Qingyin 2 ; Wang, Weiji 2 ; Meng, Xianhong 2 ;

作者机构： 1.Huaihai Inst Technol, Ocean & Fisheries Coll, Lianyungang 222005, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： C-type lectin-like protein;Fenneropenaeus chinensis;WSSV challenge;Antimicrobial activity

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A C-type lectin-like protein was cloned and characterized from the Chinese shrimp Fenneropenaeus chinensis, and named as FcCTL. The results indicated that the full length cDNA of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved Cys and one key motif EPGD. The theoretical molecular weight and pI of mature protein was 25.3 kDa and 5.4. Sequence comparison of the deduced amino acid sequence of FcCTL showed varied identity of 26–34, 34, 31 and 30 % with those of F. chinensis, Portunus trituberculatus, Tetraodon nigroviridis, Penaeus monodon, respectively. qRT-PCR analysis indicated that FcCTL was expressed highest in hepatopancreas of normal shrimp, and it’s expression was up-regulated in hepatopancreas and gills post white spot syndrome virus challenge. The purified recombinant FcCTL showed higher antimicrobial activity against Gram-positive bacteria than against Gram-negative bacteria and fungi. And the hemagglutinating activity of rFcCTL could be completely inhibited by GlcNAc (5 μg/ml), LPS (2.5 μg/ml), d-galactose (100 mM) and maltose (100 mM). These data suggested that FcCTL might play an important role in shrimp immune and would be helpful to better understandthe innate immunity mechanism of shrimp.

  • 相关文献

[1]Genomic organization and promoter characterization of FcCTL, a C-type lectin-like protein in Fenneropenaeus chinensis. Lai, XiaoFang,Gao, Huan,Yan, Binlun,Lai, XiaoFang,Gao, Huan,Kong, Jie,Wang, QingYin,Meng, XianHong,Chen, Baiyao.

[2]Genetic variation and evolutionary demography of Fenneropenaeus chinensis populations, as revealed by the analysis of mitochondrial control region sequences. Kong, Xiao Yu,Li, Yu Long,Li, Yu Long,Shi, Wei,Kong, Jie. 2010

[3]Transcriptic analysis of Huanghai No. 1 strain of Chinese shrimp Fenneropenaeus chinensis using 454 pyrosequencing. He, Yuying,Liu, Ping,Wang, Qingyin,Li, Jian,Li, Zhaoxia.

[4]Genetic variation analysis across six life periods in a natural population of the Chinese shrimp "Fenneropenaeus chinensis" in Bohai bay using SSR markers. Zhang, Kai,Wang, Weiji,Jin, Xianshi,Zhang, Kai,Xiao, Guangxia,Song, Wenping,Gao, Yan.

[5]A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis. Meng, Xian-Hong,Jang, In-Kwon,Meng, Xian-Hong,Seo, Hyung-Chul,Cho, Yeong-Rok,Kim, Bong-Rae,Ayyaru, Gopalakannan,Kim, Jong-Sheek.

[6]Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Zhang, Tianshi,Kong, Jie,Wang, Weiji,Wang, Qingyin,Zhang, Tianshi.

[7]Comparison of white spot syndrome virus quantification of fleshy shrimp Fenneropenaeus chinensis in outdoor ponds between different growing seasons by TaqMan real-time polymerase chain reaction. Meng, Xian-Hong,Jang, In Kwon,Kim, Jong-Sheek,Kim, Bong-Rae,Meng, Xian-Hong. 2011

[8]Estimation of genetic parameters and breeding values in shrimp Fenneropenaeus chinensis using the REML/BLUP procedure. Zhang Tianshi,Kong Jie,Luan Sheng,Wang Qingyin,Luo Kun,Zhang Tianshi,Tian Yi. 2011

[9]Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping. Dong Shirui,Kong Jie,Zhang Qingwen,Liu Ping,Meng Xianhong,Wang Rucai. 2006

[10]Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp I Fenneropenaeus chinensis in response to viral and bacterial infections. He, Yuying,Liu, Ping,Li, Jian,Wang, Qingyin,He, Yuying,Li, Jian,Yao, Wanlong. 2018

[11]Parentage determination of Chinese shrimp (Fenneropenaeus chinensis) based on microsatellite DNA markers. Dong, Shirui,Kong, Jie,Zhang, Tianshi,Meng, Xianhong,Wang, Rucai. 2006

[12]Analysis of DNA methylation in different tissues of Fenneropenaeus chinensis from the wild population and Huanghai No. 1. He Yuying,Li Jian,Liu Ping,Wang Qingyin,He Yuying,Li Jian,Du Ying,Li Zhaoxia. 2015

[13]Molecular cloning and characterization of a cathepsin B gene from the Chinese shrimp Fenneropenaeus chinensis. Li, Xupeng,Kong, Jie,Li, Xupeng,Meng, Xianhong,Kong, Jie,Luo, Kun,Luan, Sheng,Cao, Baoxiang,Liu, Ning,Pang, Jinfei,Shi, Xiaoli. 2013

[14]The effects of dissolved oxygen concentration and stocking density on growth and non-specific immunity factors in Chinese shrimp, Fenneropenaeus chinensis. Li, Yuquan,Li, Jian,Wang, Qingyin. 2006

[15]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

[16]Polymorphic analysis of microsatellite DNA in wild populations of Chinese shrimp (Fenneropenaeus chinensis). Liu, P,Meng, XH,Kong, J,He, YY,Wang, QY. 2006

[17]Genetic evaluation of feed efficiency in the breeding population of Fenneropenaeus chinensis "Huanghai No. 2" using phenotypic, pedigree and genomic information. Dai, Ping,Luan, Sheng,Lu, Xia,Luo, Kun,Cao, Baoxiang,Meng, Xianhong,Kong, Jie,Dai, Ping,Luan, Sheng,Lu, Xia,Luo, Kun,Cao, Baoxiang,Meng, Xianhong,Kong, Jie. 2017

[18]Influence of white spot syndrome virus infection on hepatopancreas gene expression of 'Huanghai No. 2' shrimp (Fenneropenaeus chinensis). Meng, Xianhong,Shi, Xiaoli,Kong, Jie,Luan, Sheng,Luo, Kun,Cao, Baoxiang,Liu, Ning,Lu, Xia,Li, Xupeng,Deng, Kangyu,Cao, Jiawang,Zhang, Yingxue,Zhang, Hengheng,Meng, Xianhong,Shi, Xiaoli,Kong, Jie,Luan, Sheng,Luo, Kun,Cao, Baoxiang,Liu, Ning,Lu, Xia,Li, Xupeng,Deng, Kangyu,Cao, Jiawang,Zhang, Yingxue,Zhang, Hengheng. 2017

[19]Nitrogen and phosphorus budget of a polyculture system of sea cucumber (Apostichopus japonicus), jellyfish (Rhopilema esculenta) and shrimp (Fenneropenaeus chinensis). Li Junwei,Dong Shuanglin,Gao Qinfeng,Li Junwei,Zhu Changbo. 2014

[20]Cloning of Hsp21 gene and its expression in Chinese shrimp Fenneropenaeus chinensis in response to WSSV challenge. Gao, Huan,Lai, Xiaofang,Yan, Binlun,Gao, Huan,Kong, Jie,Wang, Weiji,Meng, Xianhong,Cai, Shengli. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台