Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)
文献类型: 外文期刊
作者: Ma, Qian 1 ; Su, Yong-Quan 2 ; Wang, Jun 2 ; Zhuang, Zhi-Meng 1 ; Tang, Qi-Sheng 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Fishery Resources & Ecoenvironm, Qingdao 266071, Peoples R China
2.Xiamen Univ, Coll Oceanog & Environm Sci, Xiamen 361005, Peoples R China
关键词: Chiloscyllium plagiosum;MHC IIB;Sequence analysis;mRNA expression;Vibrio harveyi
期刊名称:FISH PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:2.794; 五年影响因子:2.876 )
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收录情况: SCI
摘要: Major histocompatibility complex (MHC) plays an important role in the immune response to antigenic peptides in vertebrates. In this study, the full length of MHC IIB cDNA was isolated from the Whitespotted bambooshark (Chiloscyllium plagiosum) by homology cloning, and the rapid amplification of cDNA ends polymerase chain reaction. As a result, the MHC IIB cDNA is 1,407 bp, which contains an open reading frame (ORF) of 831 bp encoding a protein of 276 amino acids. Furthermore, seven alleles of the complete MHC IIB ORF were detected and the variable sites were mainly located in the immunoglobulin-like (beta(2)) region. Tissue distribution analysis showed that MHC IIB can be detected in all the ten tissues examined, with the highest expression in the spleen and gill. Challenge of C. plagiosum with the pathogenic bacteria, Vibrio harveyi, resulted in significant changes in the expression of MHC IIB mRNA in the three immune-related tissues (gill, liver and spleen). These results show that the MHC IIB plays an important role in response to bacterial infection in elasmobranches.
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