Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Fusarium oxysporum f. sp cubense race 4
文献类型： 外文期刊
作者： Li, Benjin 1 ; Du, Jialiang 1 ; Lan, Chengzhong 1 ; Liu, Peiqing 1 ; Weng, Qiyong 1 ; Chen, Qinghe 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350003, Peoples R China
关键词： Fusarium oxysporum f. sp. cubense race 4;Loop-mediated isothermal amplification (LAMP);PCR;Sequence-characterised amplified region (SCAR)
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
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收录情况： SCI
摘要： Fusarium wilt (Panama disease), caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (Foc race 4), is one of the most destructive diseases affecting banana (Musa). Early and accurate detection of Foc race 4 is essential to protect the banana industry. We developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the detection of Foc race 4 based on a SCAR marker sequence. The detection limit for this assay was 10 fg per 25 mu l reaction in pure culture and DNA amplification was completed within 60 min. The assays detected 69 different isolates of Foc race 4 from geographically distinct counties in China, and no cross-reaction was observed with other fungal pathogens. When 26 infected and eight healthy looking but infested banana samples naturally from different fields were examined, the detection rate of LAMP was 100 %. The LAMP assay developed in this study was simple, fast, sensitive, and specific, and can be used in the field to detect Foc race 4 in infected banana plant tissue in resource-poor settings.
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