您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A one-step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain

文献类型： 外文期刊

作者： Zeng, W. W. 1 ; Wang, Q. 1 ; Wang, Y. Y. 1 ; Xu, D. H. 2 ; Wu, S. Q. 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China

2.ARS, Aquat Anim Hlth Res Lab, Auburn, AL 36832 USA

关键词： diagnosis technique;GCRV;RT-LAMP;segment 6 gene

期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Six reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primers designed against conserved regions of segment 6 (s6) gene were used for the detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 subtype. The entire amplification could be completed within 40 min at 62 center dot 3 degrees C. The RT-LAMP showed higher sensitivity than reverse-transcription polymerase chain reaction (RT-PCR). The RNA detection limit was 10 copies mu l1 for RT-LAMP assay and 100 copies mu l1 for conventional RT-PCR. In specificity tests, no cross-reactivity was detected in other viruses from common aquatic animals. In addition, the reaction results can be visualized by using calcein fluorescent dye. Furthermore, a total of 86 samples were tested by RT-LAMP, RT-PCR and virus isolation. The results demonstrated that all 54 specimens identified as positive by virus isolation were also positive when detected by RT-LAMP. Seven out of 54 samples, however, were misidentified by RT-PCR. The RT-LAMP method is more accurate than conventional RT-PCR. The results indicate that RT-LAMP has potential as a simple and rapid diagnosis technique for the detection of GCRV HZ08 subtype infection.

  • 相关文献

[1]Development of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of grass carp reovirus. Zhang, Qing-Li,Yan, Yi,Shi, Cheng-Yin,Wang, Qin-Tao,Huang, Jie,Shen, Jin-Yu,Hao, Gui-Jie,Liu, Hong. 2013

[2]重组GCRV-VP6枯草杆菌芽孢口服免疫对草鱼肝肠功能影响. 边青,曾伟伟,李蔚筠,池雪静,张震,赵晨峰,黄艳,余新炳. 2020

[3]草鱼jam-a分子在胚胎幼鱼期及受GCRV感染PSF细胞的表达分析. 田园园,焦真真,孙成飞,董浚键,江小燕,胡婕,叶星. 2019

[4]稳定表达草鱼LamR鱼类细胞的建立及对基因Ⅱ型GCRV增殖的影响. 黄志深,王庆,吴斯宇,周文礼,王英英,尹纪元,李莹莹. 2021

[5]Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration. Liang, Hong-Ru,Li, Yong-Gang,Zeng, Wei-Wei,Wang, Ying-Ying,Wang, Qing,Wu, Shu-Qin,Li, Yong-Gang. 2014

[6]The distribution of different virulence grass carp reovirus strains in some neglected tissues. Liang, H. R.,Fu, X. Z.,Li, N. Q.,Liu, L. H.,Lin, Q.,Li, Y. G.,Peng, Y. A.,Huang, Z. B.,Wu, S. Q.,Liang, H. R.,Li, N. Q.,Liu, L. H.,Lin, Q..

[7]A one-step duplex rRT-PCR assay for the simultaneous detection of grass carp reovirus genotypes I and II. Zeng, Weiwei,Wang, Yingying,Liang, Hongru,Liu, Cun,Song, Xinjian,Shi, Chunbin,Wu, Shuqin,Wang, Qing.

[8]七带石斑鱼神经坏死病毒的RT-LAMP检测方法. 刘滨,刘新富,曾霖,孟振,刘江春. 2016

[9]鱼类传染性造血器官坏死病毒RT-LAMP检测方法的建立及应用. 刘淼,徐黎明,卢彤岩,赵景壮,曹永生,刘红柏,尹家胜,张金凤,冯剑. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台