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The catalytic subunit of cAMP-dependent protein kinase A StPKA-c contributes to conidiation and early invasion in the phytopathogenic fungus Setosphaeria turcica

文献类型： 外文期刊

作者： Shen, Shen 1 ; Hao, Zhimin 1 ; Gu, Shouqin 1 ; Wang, Jingjing 1 ; Cao, Zhiyan 1 ; Li, Zhiyong 2 ; Wang, Qian 3 ; Li, Po 4 ;

作者机构： 1.Agr Univ Hebei, Mycotoxin & Mol Plant Pathol Lab, Baoding, Hebei Province, Peoples R China

2.Agr Acad Hebei Prov, Millet Inst, Shijiazhuang, Hebei Province, Peoples R China

3.Garden Subdist Off Qiaodong, Zhangjiakou, Hebei Province, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding, Hebei Province, Peoples R China

5.Peoples Congress Standing Comm Shijiazhuang City, Shijiazhuang, Hebei Province, Peoples R China

关键词： Melanin biosynthesis;Pathogenesis;Protein kinase A;Setosphaeria turcica;Xylanase

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

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收录情况： SCI

摘要： Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) is an important mediator of signal transduction in eukaryotic cells. Thus, identifying its function is necessary to understand the cAMP signaling network. StPKA-c, the PKA catalytic subunit gene in Setosphaeria turcica, was investigated by RNA interference technology. Transformant strains M3, M5, and M9 with diverse StPKA-c silencing efficiency were confirmed by reverse transcription polymerase chain reaction and Northern blot. Compared with the wild-type strain 01-23, the transformant strains exhibited increased growth rate and significantly decreased conidium production. In addition, the ratios of spore germination and appressorium formation and penetration were slightly reduced. Relative to the wild-type strain, the transformants demonstrated different colony color, greatly reduced pathogenicity, and similar HT-toxin activity. Further studies showed that the content of intracellular melanin in the transformants significantly decreased, and the transcription of transcriptional factor StMR was down-regulated correspondingly. The transcription and enzyme activity of xylanase was also impaired. Thus, we proposed that StPKA-c was mainly involved in the mycelium growth, conidiation, and pathogenesis of S.?turcica. Furthermore, it was positively correlated with the biosyntheses of melanin and xylanase but dispensable for the activity of HT-toxin.

  • 相关文献

[1]Molecular characterization of StpkaC2 and expression patterns of both PKA-c isoforms during the invasive growth of Setosphaeria turcica. Hao, Zhimin,Tong, Yameng,Han, Yue,Wu, Di,Yang, Zheng,Shen, Shen,Gong, Xiaodong,Cao, Zhiyan,Gu, Shouqin,Dong, Jingao,Li, Zhiyong.

[2]Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior. Gu Shou-qin,Yang Yang,Zhang Chang-zhi,Fan Yu,Zhang Xiao-yu,Tian Lan,Hao Zhi-min,Cao Zhi-yan,Gong Xiao-dong,Han Jian-min,Dong Jin-gao,Li Po,Fan Yong-shan. 2013

[3]MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica. Li Po,Gong Xiao-dong,Jia Hui,Fan Yong-shan,Zhang Yun-feng,Han Jian-min,Gu Shou-qin,Dong Jin-gao,Li Po,Fan Yong-shan,Zhang Yun-feng. 2016

[4]StSTE12 is required for the pathogenicity of Setosphaeria turcica by regulating appressorium development and penetration. Gu, Shou-qin,Li, Po,Wu, Min,Hao, Zhi-min,Gong, Xiao-dong,Zhang, Xiao-yu,Tian, Lan,Zhang, Pan,Wang, Yue,Cao, Zhi-yan,Han, Jian-min,Dong, Jin-gao,Li, Po,Fan, Yong-shan.

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