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Influence of minor displacements in loops of the porcine parvovirus VP2 capsid on virus-like particles assembly and the induction of antibody responses

文献类型： 外文期刊

作者： Pan, Qunxing 1 ; He, Kongwang 1 ; Wang, Yongshan 1 ; Wang, Xiaoli 1 ; Ouyang, Wei 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： Deletion;Displacements;Loops;PPV;Virus-like particles

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

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收录情况： SCI

摘要： An antigen-delivery system based on hybrid virus-like particles (VLPs) formed by the self-assembly of the capsid VP2 protein of porcine parvovirus (PPV) and expressing foreign peptides offers an alternative method for vaccination. In this study, the three-dimensional structure of the PPV capsid protein and surface loops deletion mutants were analyzed to define essential domains in PPV VP2 for the assembly of VLPs. Electron microscopic analysis and SDS-PAGE analysis confirmed the presence of abundant VLPs in a loop2 deletion mutant of expected size and appropriate morphology. Loop4 and loop2-loop4 deletion mutants, however, resulted in a lower number of particles and the morphology of the particles was not well preserved. Furthermore, the green fluorescent protein (gfp) gene was used as a model. GFP was observed at the same level in displacements mutants. However, GFP displacement mutants in loop2 construct allowed better adaptation for the fusion GFP to be further displayed on the surface of the capsid-like structure. Immunogenicity study showed that there is no obvious difference in mice inoculated with rAd-VP2(Δloop2), rAd-VP2(Δloop4), rAd-VP2(Δloop2-Δloop4), and PPV inactivated vaccine. The results suggested the possibility of inserting simultaneously B and T cell epitopes in the surface loop2 and the N-terminus. The combination of different types of epitopes (B, CD4+, and CD8+) in different positions of the PPV particles opens the way to the development of highly efficient vaccines, able to stimulate at the same time the different branches of the immune system.

  • 相关文献

[1]Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2. Pan, Qunxing,He, Kongwang,Pan, Qunxing,Huang, Kehe. 2008

[2]Insertions/Deletions-Associated Nucleotide Polymorphism in Arabidopsis thaliana. Guo, Changjiang,Wang, Long,Yang, Sihai,Tian, Dacheng,Du, Jianchang,Mauricio, Rodney,Gu, Tingting,Gu, Tingting. 2016

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[4]Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses. Wu, Peipei,Lu, Jihu,Zhang, Xuehua,Mei, Mei,Feng, Lei,Hou, Jibo,Tang, Yinghua,Wu, Peipei,Lu, Jihu,Zhang, Xuehua,Mei, Mei,Feng, Lei,Hou, Jibo,Tang, Yinghua,Wu, Peipei,Lu, Jihu,Zhang, Xuehua,Mei, Mei,Feng, Lei,Peng, Daxin,Hou, Jibo,Liu, Xiufan,Tang, Yinghua,Peng, Daxin,Liu, Xiufan,Kang, Sang-Moo. 2017

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