您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of Cell Types and Transcriptome Landscapes of Porcine Epidemic Diarrhea Virus-Infected Porcine Small Intestine Using Single-Cell RNA Sequencing

文献类型： 外文期刊

作者： Fan, Baochao 1 ; Zhou, Jinzhu 1 ; Zhao, Yongxiang 1 ; Zhu, Xuejiao 1 ; Zhu, Mingjun 1 ; Peng, Qi 1 ; Li, Jizong 1 ; Chang, Xinjian 1 ; Shi, Danyi 1 ; Yin, Jie 1 ; Guo, Rongli 1 ; Li, Yunchuan 1 ; He, Kongwang 1 ; Fan, Huiying 3 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Inst Vet Med,Key Lab Vet Biol, Nanjing, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

3.Jiangsu Univ, Sch Life Sci, Zhenjiang, Peoples R China

4.South China Agr Univ, Coll Vet Med, Guangzhou, Peoples R China

5.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China

6.Jiangsu Acad Agr Sci, Inst Vet Med, 50 Zhong Ling St, Nanjing 210014, Peoples R China

期刊名称：JOURNAL OF IMMUNOLOGY （ 影响因子：4.4； 五年影响因子：5.2 ）

ISSN： 0022-1767

年卷期： 2023 年 210 卷 3 期

页码：

收录情况： SCI

摘要： Swine coronavirus-porcine epidemic diarrhea virus (PEDV) with specific susceptibility to pigs has existed for decades, and recurrent epidemics caused by mutant strains have swept the world again since 2010. In this study, single-cell RNA sequencing was used to perform for the first time, to our knowledge, a systematic analysis of pig jejunum infected with PEDV. Pig intestinal cell types were identified by representative markers and identified a new tuft cell marker, DNAH11. Excepting enterocyte cells, the goblet and tuft cells confirmed susceptibility to PEDV. Enrichment analyses showed that PEDV infection resulted in upregulation of cell apoptosis, junctions, and the MAPK signaling pathway and downregulation of oxidative phosphorylation in intestinal epithelial cell types. The T cell differentiation and IgA production were decreased in T and B cells, respectively. Cytokine gene analyses revealed that PEDV infection downregulated CXCL8, CXCL16, and IL34 in tuft cells and upregulated IL22 in Th17 cells. Further studies found that infection of goblet cells with PEDV decreased the expression of MUC2, as well as other mucin components. Moreover, the antimicrobial peptide REG3G was obviously upregulated through the IL33-STAT3 signaling pathway in enterocyte cells in the PEDV-infected group, and REG3G inhibited the PEDV replication. Finally, enterocyte cells expressed almost all coronavirus entry factors, and PEDV infection caused significant upregulation of the coronavirus receptor ACE2 in enterocyte cells. In summary, this study systematically investigated the responses of different cell types in the jejunum of piglets after PEDV infection, which deepened the understanding of viral pathogenesis. The Journal of Immunology, 2023,210: 271-282.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台