Cloning and characterization of ubiquitin ribosome fusion gene RpS27a, a deltamethrin-resistance-associated gene from diamondback moth (Plutella xylostella L.)
文献类型： 外文期刊
作者： Zhang, Hong 1 ; Cheng, Chen 1 ; Li, Fengliang 2 ; Gu, Shuyu 1 ; Zhou, Zhou 1 ; Cheng, Luogen 1 ;
作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing, Jiangsu, Peoples R China
2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China
关键词： cDNA cloning;Insecticide resistance;Plutella xylostella;Real-time quantitative PCR;Ubiquitin ribosome fusion gene
期刊名称：TURKISH JOURNAL OF ZOOLOGY （ 影响因子：0.673； 五年影响因子：0.784 ）
ISSN：
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收录情况： SCI
摘要： The ubiquitin ribosome fusion gene RpS27a's coding sequence was cloned from diamondback moth, Plutella xylostella (L.). An open reading frame of 468 bp was found to encode a putative 155 amino acid precursor protein, which shares over 90% similarity with other insects' RpS27a protein. Real-time quantitative PCR was carried out to determine the relative expression level in the fourth instar larvae of deltamethrin-resistant and deltamethrin-susceptible strains. Te results showed that the expression of RpS27a was significantly higher in the deltamethrin-resistant strain than in the deltamethrin-susceptible strain. There are reports indicating that upregulation expression of some ribosomal proteins confers some insecticide resistance. For the first time, we have predicted that the increased expression of the RpS27a gene may have some association with pesticide resistance in Plutella xylostella.
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