Transgenic expression of an insect diapause-specific peptide (DSP) in Arabidopsis resists phytopathogenic fungal attacks
文献类型： 外文期刊
作者： Wu, Tingquan 1 ; Chen, Yongfang 2 ; Chen, Weida 2 ; Zou, Shenghao 2 ; Zhang, Yongqing 2 ; Lin, Yu'e 1 ; Liang, Zhaojun 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China
2.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
关键词： Diapause-specific peptide;Antimicrobial peptide;Phytopathogenic fungus;Transgenic expression
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
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收录情况： SCI
摘要： Diapause-specific peptide (DSP) is an insect antimicrobial peptide, composed of 41 amino acid residues including six cysteines and isolated from diapausing adults of the leaf beetle Gastrophysa atrocyaneais. Recent research results have been demonstrated that in vitro, DSP has selective antifungal activity against the higher animal pathogenic fungus Trichophyton rubrum and the plant phytopathogenic fungus Fusarium solani. To elucidate the effect of DSP on other phytopathogenic fungi, we respectively introduced a Cht1SP-DSP-FLAG fusion gene and Cht1SP-FLAG fusion gene into Arabidopsis ecotype col-0. Transgenic plants expressing Cht1SP-DSP-FLAG fusion gene showed significant resistance to Golovinomyces cichoracearum (biotrophic fungus), Botrytis cinerea (necrotrophic fungus) and Hyaloperonospora arabidopsidis (H. a.) Noco2 (oomycete), whilst transgenic plants expressing Cht1SP-FLAG fusion gene showed no resistance to these pathogens. These results indicated that for many plant pathogenic fungi, DSP has high antimicrobial activity, which suggests that DSP has a huge potential in protecting crops from damage from phytopathogenic fungi.
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