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Gonadogenesis and Expression Pattern of the vasa Gene in the Sea Cucumber Apostichopus japonicus During Early Development

文献类型： 外文期刊

作者： Yu, Li 1 ; Yan, Meng 1 ; Sui, Juan 2 ; Sheng, Wan-Qiang 3 ; Zhang, Zhi-Feng 1 ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Qingdao, Peoples R China

3.Natl Univ Singapore, Dept Biol Sci, Kent Ridge, Singapore

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

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收录情况： SCI

摘要： Vasa has been extensively used as a germ-line marker to trace the origin and migration pathway of primordial germ cells (PGCs) in many organisms, but little work has been reported on vasa genes and the origin of PGCs in holothurians. Using in situ hybridization and immunohistochemistry, vasa mRNA and protein of the sea cucumber Apostichopus japonicus (Aj-vasa) was detected in the cytoplasm of the unfertilized egg and was equally distributed in the cytoplasm of early embryos, from the two-cell embryoto the blastula, indicating that Aj-vasa mRNA is maternally supplied. Later, expression of both Aj-vasa mRNA and protein centralizes gradually in newly organized structures from blastula to five-tentacle larva, and then is restricted to PGC-like cells ofthe original gonad in juveniles with 0.1-cm body length. The structure of the gonad develops further from a simple tubular gonad in 0.5-cm-length juveniles to a branched gonad in 3-cm-length juveniles. Our findings showed that the maternal supply of thevasa gene products in A. japonicus is different from that in sea urchin Strongylocentrotus purpuratus, of echinoderm, and suggested that the specialization of PGCs is an epigenesis mechanism in A. japonicus.

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