文献类型： 外文期刊
作者： Chen, Ling 1 ; Miao, Yingjie 2 ; Wang, Cheng 2 ; Su, Peipei 2 ; Li, Tianheng 3 ; Wang, Rong; Hao, Xinglong; Yang, G 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Food Crops, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Peoples R China
2.Huazhong Univ Sci & Technol HUST, Genet Engn Int Cooperat Base, China UK HUST RRes Genet Engn & Genom Joint Lab, Coll Life Sci & Technol,Minist Sci & Technol,Wuha, Wuhan 430074, Peoples R China
3.Huazho
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
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收录情况： SCI
摘要： PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.
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