Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice
文献类型： 外文期刊
作者： Zhao, Feng 1 ; Li, Qiuying 2 ; Weng, Manli 3 ; Wang, Xiuliang 2 ; Guo, Baotai 4 ; Wang, Li 3 ; Wang, Wei 3 ; Duan, Delin; 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
4.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词： Agrobacterium-mediated transformation;Salt-tolerance;Transgenic rice;Trehalose-6-phosphate synthase (TPS);Zostera marina
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： The full-length cDNA sequence (2613bp) of the trehalose-6-phosphate synthase (TPS) gene of eelgrass Zostera marina (ZmTPS) was identified and cloned. Z. marina is a kind of seed-plant growing in sea water during its whole life history. The open reading frame (ORF) region of ZmTPS gene encodes a protein of 870 amino acid residues and a stop codon. The corresponding genomic DNA sequence is 3770bp in length, which contains 3 exons and 2 introns. The ZmTPS gene was transformed into rice variety ZH11 via Agrobacterium-mediated transformation method. After antibiotic screening, molecular characterization, salt-tolerance and trehalose content determinations, two transgenic lines resistant to 150mM NaCL solutions were screened. Our study results indicated that the ZmTPS gene was integrated into the genomic DNA of the two transgenic rice lines and could be expressed well. Moreover, the detection of the transformed ZmTPS gene in the progenies of the two transgenic lines was performed from T1 to T4 generations; and results suggested that the transformed ZmTPS gene can be transmitted from parent to the progeny in transgenic rice.
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