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Identification and mRNA expression profile of glutamate receptor-like gene in quinclorac-resistant and susceptible Echinochloa crus-galli

文献类型： 外文期刊

作者： Li, Gang 1 ; Wu, Shenggan 1 ; Cai, Leiming 1 ; Wang, Qiang 1 ; Zhao, Xueping 2 ; Wu, Changxing;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Pesticide Residue Detect &, Agr Minist,Key Lab Pestidde Residue Detect,Inst Q, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Pesticide Residue Detect &, Agr Minist,Key Lab Pestidde R

关键词： Barnyardgrass;Glutamate receptor-like genes (GLR);Quinclorac-response gene

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： Animal ionotropic glutamate receptors (iGluRs) function as Ca2+ ion channels during excitatory neurotransmission in nerve cells. Here, a glutamate receptor-like gene (GLR) was identified and characterized from a plant - Echinochloa crus-galli. The GLR gene was designated EcGLR1 with GenBank no: JX518597. It has a 2793bp open reading frame predicted to encode a 101.7kDa protein. Sequence alignment showed that EcGLR1 is a GLR homologue. Its expression in response to quinclorac treatment was assessed by real-time PCR in near-isogenic lines of quinclorac-resistant (R) and susceptible (S) biotypes of E. crus-galli. The expression of EcGLR1 in the seedling leaf and root at least increased 5 times in the S plants and 22 times in the R plants after exposure to quinclorac. In the adult plant leaves, roots and stems, its expression increased 11-14 times in the S plants and 23-25 times in the R plants after quinclorac stimulation. In the seed, its expression was 4 times less in the S plants than that in the R plants, but after treatment, the levels all increased by about 24 times in the two biotypes. EcGLR1 expression was 1-4 times greater in the R plants than in that in the S plants, and after treatment by quinclorac, the difference increased to a ratio of 4 to 9. Its expression was higher in all tissues tested of R biotypes than in that of S plants before or after quinclorac treatment. The results of this study provide basic information for the further research of function of the EcGLR1 in resistance to quinclorac in E. crus-galli.

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[1]Identification and expression pattern of a glutathione S-transferase in Echinochloa crus-galli. Li, G.,Wu, S. G.,Yu, R. X.,Cang, T.,Chen, L. P.,Zhao, X. P.,Cai, L. M.,Wu, C. X.. 2013

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