Expression of biologically active human recombinant interferon alpha 2b in human breast cancer cell line Bcap-37
文献类型： 外文期刊
作者： Li, Hui 1 ; Li, Xiangping 2 ; Liu, Qingyou 2 ; Shi, Zhendan 1 ; Shi, Deshun 2 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China
2.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China
关键词： Bcap-37;Biological active;IFN-inducible genes;Interferon alpha 2b
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Human interferon alpha 2b (IFNα-2b) is a pleiotropic cytokine used to treat various viral diseases and cancers. Conventionally, recombinant human IFNα-2b used in clinics was produced by prokaryotic expression system, which always lack of enough biological activity due to limitations on proper folding and post-translational modifications, so the eukaryotic expression system are becoming prevailing method for the production of recombinant proteins. In this study, human breast cancer cell Bcap-37 was firstly used as host for the expression of human IFNα-2b, with the expression vector pIRES2-IFN-EGFP, in which IFNα-2b gene is under the control of CMV promoter. The expression of recombinant IFNα-2b was detected by Western blot and ELISA. Results showed that the concentration of the secreted recombinant IFNα-2b in culture medium was 435.7 pg/mL/24 h. Biological activity of the recombinant IFNα-2b was assayed by detecting the expression of IFN-inducible genes, including MxA, OAS, PKR, and Caspase1 through QRT-PCR. Results demonstrated that recombinant IFNα-2b possess the biological activities. Compared to non-transgenic cells, the expression levels of the aforementioned four IFN-inducible genes were increased by 18.098-, 1.843-, 2.21-, and 3.066-folds, respectively. We got to a conclusion that the human breast cancer cell Bcap-37 could express bioactive recombinant IFNα-2b.
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