您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Proteomic identification of gravitropic response genes in peanut gynophores

文献类型： 外文期刊

作者： Li, Hai-Fen 1 ; Zhu, Fang-He 1 ; Li, He-Ying 2 ; Zhu, Wei 1 ; Chen, Xiao-Ping 1 ; Hong, Yan-Bin 1 ; Liu, Hai-Yan 1 ; Wu, 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Crops Res Inst, Guangzhou 510640, Peoples R China

2.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China

关键词： Gravitropism;Gynophore;Peanut;Proteome

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Peanut (Arachis hypogaea L.) is one of the most important oil-bearing crops in the world. The gravitropic response of peanut gynophores plays an essential role in peanut reproductive development. In this study, we developed an in vitro culture system and applied it to the study of peanut gynophore gravitropism. By comparing the proteomes of gynophores grown in vitro with the tip pointing upward (gravity stimulation sample) and downward (natural growth control) at 6. h and 12. h, we observed 42 and 39 with significantly altered expression pattern at 6 and 12. h, respectively. Out of these proteins, 13 proteins showed same expression profiling at both 6. h and 12. h. They were identified by MALDI-TOF/TOF and further characterized with quantitative real time RT-PCR. Among the 13 identified proteins, two were identified as class III acidic endochitinases, two were identified as Kunitz trypsin protease inhibitors, and the remaining proteins were identified as pathogenesis-related class 10 protein, Ara h 8 allergen isoform 3, voltage-dependent anion channel, gamma carbonic anhydrase 1, germin-like protein subfamily 3 member 3 precursor, chloride channel, glycine-rich RNA-binding protein and gibberellin receptor GID1. Real time RT-PCR analysis revealed that transcriptional regulation is consistent with expression at the protein level for class III acidic endochitinase, Kunitz trypsin protease inhibitor, chloride channel and pathogenesis-related class 10 protein, while the expression of the other 7 proteins might be regulated at post-transcriptional levels. This study identified several potential gravitropic response proteins in peanut gynophores and helps to understand early gravitropic responses in peanut gynophores. Biological significance: The gravitropic response of the peanut gynophores plays an essential role in peanut production. However, the molecular mechanism responsible for gravitropic responses in the peanut gynophores has not been explored yet. The result generated in this study may provide in vitro culture system for gravitropism study of plant gravitropic response and novel insights into the proteome-level response and give a more comprehensive understanding of early gravitropic response in peanut gynophores.This article is part of a Special Issue entitled: Translational Plant Proteomics.

  • 相关文献

[1]Transcriptome profiling of peanut (Arachis hypogaea) gynophores in gravitropic response. Li, Hai-fen,Chen, Xiao-Ping,Zhu, Fang-he,Liu, Hai-Yan,Hong, Yan-Bin,Liang, Xuan-Qiang. 2013

[2]Identification and Evaluation of Single-Nucleotide Polymorphisms in Allotetraploid Peanut (Arachis hypogaea L.) Based on Amplicon Sequencing Combined with High Resolution Melting (HRM) Analysis. Hong, Yanbin,Liu, Ying,Chen, Xiaoping,Liang, Xuanqiang,Hong, Yanbin,Huang, Shangzhi,Pandey, Manish K.,Varshney, Rajeev K.,Liu, Hong,Varshney, Rajeev K.,Varshney, Rajeev K.. 2015

[3]Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea). Chen, Xiao-Ping,Li, Hai-Fen,Liu, Hai-Yan,Hong, Yan-Bin,Liang, Xuan-Qiang,Wang, Tong,Li, Ling,Liang, Xuan-Qiang,Wang, Tong,Yang, Qing-Li,Chi, Xiao-Yuan,Yang, Zhen,Yu, Shan-Lin. 2013

[4]Phylogenetic diversity of Calonectria ilicicola causing Cylindrocladium black rot of peanut and red crown rot of soybean in southern China. Gai, Yunpeng,Deng, Qiaowen,Chen, Xinglong,Guan, Mingfang,Xiao, Xiang,Xu, Dagao,Pan, Ruqian,Gai, Yunpeng,Deng, Mingguang.

[5]MOLECULAR CHARACTERIZATION OF ARACHIS HYPOGAEA NAC 2 (AHNAC2) REVEALS IT AS A NAC-LIKE PROTEIN IN PEANUT. Liu, X.,Zhang, B. Y.,Hong, L.,Su, L. C.,Li, L.,Liang, X. Q.,Li, X. Y.. 2010

[6]Rapid Identification of Peanut Hybrids with SSR-PCR. Hong Yanbin,Li Xingyu,Huang Shangzhi,Hong Yanbin,Chen Xiaoping,Liang Xuanqiang,Liu Hong. 2015

[7]Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut. Zhu, Wei,Chen, Xiaoping,Li, Haifen,Zhu, Fanghe,Hong, Yanbin,Liang, Xuanqiang,Varshney, Rajeev K..

[8]Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid. Zhong, Weijing,Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Zhong, Weijing,Yang, Lin,Zou, Shutong. 2011

[9]Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台