Preparation of cross-linked enzyme aggregates in water-in-oil emulsion: Application to trehalose synthase
文献类型： 外文期刊
作者： Chen, Ying 1 ; Xiao, Chen-peng 2 ; Chen, Xiao-yun 1 ; Yang, Li-wei 1 ; Qi, Xin 1 ; Zheng, Jian-feng 2 ; Li, Ming-chun; 1 ;
作者机构： 1.Tianjin Acad Agr Sci, Tianjin Inst Forestry & Pomol, Tianjin, Peoples R China
2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
关键词： Trehalose synthase;Spherical cross-linked enzyme aggregates;Water-in-oil emulsion
期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： In this work, a novel method to prepare cross-linked enzyme aggregates (CLEA) in a water-in-oil emulsion was reported. Spherical CLEA of recombinant trehalose synthase (TreS) from Meiothermus rubber was obtained through the emulsion based process. By varying the experimental parameters, the optimum conditions for the preparation of spherical CLEA were determined. The optimum catalytic temperature and thermal stability of spherical CLEA were enhanced significantly compared with the free enzyme. Spherical CLEA was also more tolerant to metal ions and chemical denaturants. Optical and scanning electron microscopy analysis showed that instead of the amorphous clumping shape of conventionally prepared CLEA, spherical CLEA has a more structured ball-like appearance with a size of 20-60 μm in diameter that could be easily separated.
- 相关文献
作者其他论文 更多>>
-
Computation-Directed Molecular Design, Synthesis, and Fungicidal Activity of Succinate Dehydrogenase Inhibitors
作者：Li, Kun;Hong, Shuang;Yu, Zhenwu;Hong, Zeyu;Sun, Yaru;Tang, Liangfu;Fan, Zhijin;Li, Kun;Hong, Shuang;Yu, Zhenwu;Hong, Zeyu;Sun, Yaru;Tang, Liangfu;Fan, Zhijin;Cheng, Jiagao;Wang, Yong;Qi, Xin
关键词：SDH; molecular docking; 3D-QSAR; moleculardynamics simulations; MM-GBSA; design and synthesize
-
Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation
作者：Wang, Lu;Cheng, Jianing;Bi, Shuangtian;Wang, Jinshu;Cheng, Xin;Liu, Shihang;Gao, Yue;Wang, Che;Wang, Lu;Lan, Qingkuo;Shi, Xiaowei;Wang, Yong;Zhao, Xin;Qi, Xin;Xu, Shiyong;Wang, Lu;Lan, Qingkuo;Shi, Xiaowei;Wang, Yong;Zhao, Xin;Qi, Xin;Xu, Shiyong
关键词：ADF1; MYB30; actin filaments; thermal adaptation; Arabidopsis; Chinese cabbage
-
Overexpression of MYB-like transcription factor SiMYB30 from foxtail millet (Setaria italica L.) confers tolerance to low nitrogen stress in transgenic rice
作者：Zhang, Yuewei;Chen, Kai;Wang, Chunxiao;Zhou, Yongbin;Xu, Zhaoshi;Chen, Jun;Tang, Wensi;Ma, Youzhi;Chen, Ming;He, Zhang;Guo, Changhong;Qi, Xin;Li, Maomao;Liu, Jin;Le, Si
关键词：Foxtail millet; Low nitrogen; MYB-Like transcription factor; Nitrogen use efficiency; Transgenic rice
-
Investigations on the Fusants From Wide Cross Between White-Rot Fungi and Saccharomyces cerevisiae Reveal Unknown Lignin Degradation Mechanism
作者：Shao, Qi;Li, Xin;Wei, Dongsheng;Chen, Ying;Zhang, Zhijun;Cui, Yong;Fan, Huan
关键词：lignin degradation; white-rot fungi; protoplast fusion; biomass conversion; 2D-HSQC NMR
-
Synthesis of the ternary nanocomposites composed of zinc 2-methylimidazolate frameworks, lactoferrin and melittin for antifungal therapy
作者：Yu, Dixiong;Yu, Qilin;Liu, Shuo;Li, Mingchun;Wang, Yufan;Zhang, Jun;Liu, Shuo
关键词：
-
Effect of glycosylation patterns of Chinese eggplant anthocyanins and other derivatives on antioxidant effectiveness in human colon cell lines
作者：Jing, Pu;Qian, Bingjun;Zhao, Shujuan;Ye, Ludan;Wang, Xingya;Qi, Xin;Giusti, M. Monica
关键词：Antioxidants;Delphinidin derivatives;HT-29;HCT-116;Glycosylation
-
Preparation of Cross-Linked Enzyme Aggregates of Trehalose Synthase via Co-aggregation with Polyethyleneimine
作者：Zheng, Jianfeng;Chen, Ying;Yang, Liwei;Zhang, Jun;Zheng, Jianfeng;Li, Mingchun
关键词：Trehalose synthase;Polyethyleneimine;Co-aggregation;Cross-linking
