Purification and partial characterization of a novel anti-tumor glycoprotein from cultured mycelia of Grifola frondosa
文献类型: 外文期刊
作者: Cui, Fengjie 1 ; Zan, Xinyi 1 ; Li, Yunhong 1 ; Yang, Yan 3 ; Sun, Wenjing 1 ; Zhou, Qiang 2 ; Yu, Silian 2 ; Dong, Ying 1 ;
作者机构: 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
2.Jiangxi Prov Engn & Technol Ctr Food Addit Biopro, Dexing 334221, Peoples R China
3.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
4.Parchn Sodium Isovitamin C Co Ltd, Dexing 334221, Peoples R China
关键词: Grifola frondosa;Glycoprotein;Purification;Structural characterization;Anti-tumor activity
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN:
年卷期:
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收录情况: SCI
摘要: A novel glycoprotein GFG-3a with the molecular weight of 88.01 kDa and potent anti-tumor activity was isolated from the cultured mycelia of Grifola frondosa GF9801. GFG-3a was heat-sensitive with the decreasing anti-proliferative activity after treated from 56℃ to 100℃ for 10-120 min. GFG-3a was a glycoprotein with O-glycosylation and contained 6.20% carbohydrate composed of d-arabinose, d-fructose, d-mannose, and d-glucose with a molar ratio of 1.33:4.51:2.46:1.00. FT-IR and NMR spectra proved that GFG-3a contained protein and carbohydrate portions with 3-O-methyl-galactose residues, (1→4)-linked (3-galactose residues, and β-linked glucose residues. Circular dichroism (CD) revealed that GFG-3a was a predominantly β-sheet glycoprotein with a relatively small a-helical content. Protein sequencing and 3D model of GFG-3a were finally predicted by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service. Our findings will be a reference for the further structure-activity relationship analysis of the mushroom glycoproteins.
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