Purification and partial characterization of a novel anti-tumor glycoprotein from cultured mycelia of Grifola frondosa
文献类型: 外文期刊
作者: Cui, Fengjie 1 ; Zan, Xinyi 1 ; Li, Yunhong 1 ; Yang, Yan 3 ; Sun, Wenjing 1 ; Zhou, Qiang 2 ; Yu, Silian 2 ; Dong, Ying 1 ;
作者机构: 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
2.Jiangxi Prov Engn & Technol Ctr Food Addit Biopro, Dexing 334221, Peoples R China
3.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
4.Parchn Sodium Isovitamin C Co Ltd, Dexing 334221, Peoples R China
关键词: Grifola frondosa;Glycoprotein;Purification;Structural characterization;Anti-tumor activity
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A novel glycoprotein GFG-3a with the molecular weight of 88.01 kDa and potent anti-tumor activity was isolated from the cultured mycelia of Grifola frondosa GF9801. GFG-3a was heat-sensitive with the decreasing anti-proliferative activity after treated from 56℃ to 100℃ for 10-120 min. GFG-3a was a glycoprotein with O-glycosylation and contained 6.20% carbohydrate composed of d-arabinose, d-fructose, d-mannose, and d-glucose with a molar ratio of 1.33:4.51:2.46:1.00. FT-IR and NMR spectra proved that GFG-3a contained protein and carbohydrate portions with 3-O-methyl-galactose residues, (1→4)-linked (3-galactose residues, and β-linked glucose residues. Circular dichroism (CD) revealed that GFG-3a was a predominantly β-sheet glycoprotein with a relatively small a-helical content. Protein sequencing and 3D model of GFG-3a were finally predicted by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service. Our findings will be a reference for the further structure-activity relationship analysis of the mushroom glycoproteins.
- 相关文献
作者其他论文 更多>>
-
Nanoliposome based strategy for enhancing bioavailability and antitumor activity of polyphenol from Sanghuangporus vaninii
作者:Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Liu, Junbo;Wang, Weike
关键词:Sanghuangporus vaninii; Nanoliposome; Liver tumor; Polyphenol; Bioavailability
-
Targeted isolation and AI-based analysis of edible fungal polysaccharides: Emphasizing tumor immunological mechanisms and future prospects as mycomedicines
作者:Li, Tingting;Bi, Yong;Li, Tingting;Rui, Chuang;Ren, Lu;Wang, Qin;Dai, Mingcheng;Yang, Yan
关键词:Keywords; Edible fungal polysaccharide; Structural characterization; Gut microbiota; Tumor microenvironment; Mycomedicines; Deep learning; Glycan structure prediction"
-
Novel Umami Peptides from Lentinula edodes: Evaluation of the Umami Enhancing Effect Using Biosensors and Molecular Simulation
作者:Zhao, Shilong;Cui, Zhiyong;Zheng, Yuqian;Yu, Yanyang;Zhou, Changlin;Zhou, Quanlong;Zhang, Amin;Guo, Xueqian;Liu, Yuan;Wang, Wenli;Zhao, Shilong;Wang, Shengnan;Xu, Changhua;Liu, Yuan;Yang, Yan;Luo, Zisheng
关键词:
Lentinula edodes ; umami peptide; umami enhancing effect; biosensor; moleculardynamics simulation -
The ENaC taste receptor's perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis
作者:Li, Wen;Chen, Wanchao;Wu, Di;Zhang, Zhong;Liu, Peng;Li, Zhengpeng;Yang, Yan
关键词:
-
A novel directed enzymolysis strategy for the preparation of umami peptides in Stropharia rugosoannulata and its mechanism of taste perception
作者:Chen, Daoyou;Rong, Mingli;Yuan, Zhaoting;Miao, Tingwei;Song, Hucheng;Zhang, Lujia;Yang, Yan;Zhang, Chuanxi;Wei, Hao;Tang, Shuting;Jiang, Haiming
关键词:Stropharia rugosoannulata; Directed enzymolysis; Virtual screening; Umami peptide; Molecular simulation
-
Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice
作者:Liu, Peng;Gao, Chen;Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Yang, Yan;Gao, Chen;Li, Tingzhao;Sun, Shuai;Li, Tingzhao
关键词:Stropharia rugosoannulata; Obesity; Protein; Hepatic steatosis; Preparation strategy
-
The mechanism of preharvest treatment of polysaccharide from Lentinus edodes residue on inhibiting browning of postharvest Agaricus bisporus
作者:Liu, Huiqin;Li, Wen;Liu, Peng;Chen, Wanchao;Zhang, Zhong;Yang, Yan;Liu, Huiqin;Li, Wen;Liu, Peng;Chen, Wanchao;Zhang, Zhong;Yang, Yan;Liu, Huiqin;Li, Jingjun
关键词:Polysaccharide; Preharvest treatment; Browning; Agaricus bisporus; Lentinula edodes residue



