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Molecular characterization and differential expression of multiple goose dopamine D2 receptors

文献类型： 外文期刊

作者： Wang, Cui 1 ; Liu, Yi 1 ; Wang, Huiying 1 ; Wu, Huali 1 ; Gong, Shaoming 1 ; Chen, Weihu 2 ; He, Daqian 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China

2.Zhedong White Goose Inst Xiangshan Cty, Ningbo 315700, Zhejiang, Peoples R China

关键词： Alternative splicing;Cloning;Differential expression;DRD2;SNPs

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： Dopamine D2 receptor (DRD2) gene, a member of the dopamine receptors gene family, has been studied as a candidate gene for broodiness due to its special effects on avian prolactin secretion. Here, the genomic DNA and cDNA sequences of goose (Anser cygnoides) DRD2 gene were cloned and characterized for the first time. The goose DRD2 cDNA is 1353. bp in length and encodes a protein of 450 amino acids. The length of goose DRD2 genomic DNA is 8350. bp, including seven exons and six introns. We identified four goose DRD2 variants, which were generated due to alternative splicing. Bioinformatics analysis indicates that all the deduced DRD2 amino acid sequences contain seven putative transmembrane domains and four potential N-glycosylation sites. A phylogenetic tree based on amino acid sequences displays that the goose DRD2 protein is closely related to those of avian species. Semi-quantitative RT-PCR analysis demonstrates that the DRD2-1, DRD2-2 and DRD2-4 transcripts are differentially expressed in the pituitary, ovary, hypothalamus, as well as in the kidney, whereas the DRD2-3 transcript is widely expressed in all the examined tissues at different levels. Meanwhile, 54 single nucleotide polymorphisms (SNPs) and 4 insert-deletion (indel) variations were identified in the coding region and partial intron region of the goose DRD2 gene. Those findings will help us gain insight into the functions of the DRD2 gene in geese.

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