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A Colpoda aspera isolate from animal faeces: In vitro cultivation and identification

文献类型： 外文期刊

作者： Shi, Tuanyuan 1 ; Shi, Xinlu 2 ; Lu, Fuzhuang 1 ; Yu, Fuxian 1 ; Fu, Yuan 1 ; Hu, Xiaozhong 3 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Dept Anim Parasitol, Hangzhou 310021, Zhejiang, Peoples R China

2.Hangzhou Normal Univ, Sch Life & Environm Sci, Hangzhou Key Lab Anim Sci & Technol, Hangzhou 310036, Zhejiang, Peoples R China

3.Ocean Univ China, Inst Evolut & Marine Biodivers, Lab Protozool, Qingdao 266003, Peoples R China

关键词： Colpoda aspera;Chemically defined medium;18S ribosomal RNA gene;Excystment

期刊名称：EUROPEAN JOURNAL OF PROTISTOLOGY （ 影响因子：3.02； 五年影响因子：2.708 ）

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收录情况： SCI

摘要： A colpodean ciliate was found in the faeces of experimental rabbits. It was initially cultivated in medium mixed with 2% (w/v) rabbit faeces. Subsequently, two chemically defined media, designated CA-1 and CA-2, were found to be suitable for axenical cultivation of the ciliate. The maximum abundance of the ciliate isolate in the CA media was 1-2 x 10~5 cells/ml. The ciliate isolate was further identified with silver impregnation and molecular analysis. Features of the left oral polykinetid, somatic dikinetids, and sliverline pattern were similar to those of Colpoda aspera as described by Foissner (1993). The 18S small subunit ribosomal RNA gene of the ciliate isolate shared 99% sequence identity with that of C. aspera, with 100% coverage, and formed a sister clade in the phylogenetic tree with the reference C. aspera isolate. In addition, the trophozoite of C. aspera could proliferate over a temperature range from 25-37 ℃. When resting cysts were cultivated in CA-1 medium at 30-35 ℃, 98.2% of the trophozoites were detached from the cyst wall after 7h.

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