您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of two acetylcholinesterases in Pardosa pseudoannulata and the sensitivity to insecticides

文献类型： 外文期刊

作者： Zhang, Yixi 1 ; Shao, Ying 1 ; Jiang, Feng 1 ; Li, Jian 1 ; Liu, Zewen 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

2.E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Pardosa pseudoannulata;Acetylcholinesterase;Insecticide selectivity

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Pardosa pseudoannulata is an important predatory enemy against insect pests, such as rice planthoppers and leafhoppers. In order to understand the insecticide selectivity between P. pseudoannulata and insect pests, two acetylcholinesterase genes, Pp-ace1 and Pp-ace2, were cloned from this natural enemy. The putative proteins encoded by Pp-ace1 and Pp-ace2 showed high similarities to insect AChE1 (63% to Liposcelis entomophila AChE1) and AChE2 (36% to Culex quinquefasciams AChE2) with specific functional motifs, which indicated that two genes might encode AChE1 and AChE2 proteins respectively. The recombinant proteins by expressing Pp-ace1 and Pp-ace2 genes in insect sf9 cells showed high AChE activities. The kinetic parameters,.V-max and K-m, of two recombinant AChE proteins were significantly different. The sensitivities to six insecticides were determined in two recombinant AChEs. Pp-AChE1 was more sensitive to all tested insecticides than Pp-AChE2, such as fenobucarb (54 times in K, ratios), isoprocarb (31 times), carbaryl (13 times) and omethoate (6 times). These results indicated that Pp-AChE1 might be the major synaptic enzyme in the spider. By sequence comparison of P. pseudoannulata and insect AChEs, the key amino acid differences at or close to the functional sites were found. The locations of some key amino acid differences were consistent with the point mutation sites in insect AChEs that were associated with insecticide resistance, such as Phe331 in Pp-AChE2 corresponding to Ser331Phe mutation in Myzus persicae and Aphis gossypii AChE2, which might play important roles in insecticide selectivity between P. pseudoannulata and insect pests. Of course, the direct evidences are needed through further studies

  • 相关文献

[1]Validating the importance of two acetylcholinesterases in insecticide sensitivities by RNAi in Pardosa pseudoannulata, an important predatory enemy against several insect pests. Meng, Xiangkun,Li, Chunrui,Bao, Haibo,Liu, Zewen,Zhang, Yixi,Bao, Haibo,Fang, Jichao.

[2]Cloning of Two Acetylcholinesterase Genes and Analysis of Point Mutations Putatively Associated with Triazophos Resistance in Chilo auricilius (Lepidoptera: Pyralidae). Li, Xiao-Huan,Zhang, Zhi-Chun,Liu, Bao-Sheng,Fang, Ji-Chao,Li, Xiao-Huan,Fang, Ji-Chao,Huang, Shui-Jin.

[3]Biochemistry and molecular characterisation of chlorpyrifos resistance in field strains of the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae). He, Ming,He, Peng,Ai, Zhen-Xian,Jiang, Zi-Qiong,Long, Yu-Ning,Zhang, Yue-Liang.

[4]Cross-resistance and possible mechanisms of chlorpyrifos resistance in Laodelphax striatellus (Fallen). Wang, Lihua,Zhang, Yueliang,Liu, Yanhe,Fang, Jichao,Zhang, Yueliang,Han, Zhaojun,Liu, Yanhe. 2010

[5]Overexpression of carboxylesterase-1 and mutation (F439H) of acetylcholinesterase-1 are associated with chlorpyrifos resistance in Laodelphax striatellus. Zhang, Yueliang,Li, Shuo,Guo, Hui Fang,Zi, Jinyan,Wang, Lihua,Fang, Jichao,Xu, Lu,Zi, Jinyan,He, Peng. 2013

[6]Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae). Jiang, Xiaojing,Jiang, Weihua,Han, Zhaojun,Qu, Mingjing,Denholm, Ian,Fang, Jichao. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台