Maize (Zea mays L.) transformation by Agrobacterium tumefaciens infection of pollinated ovules
文献类型： 外文期刊
作者： Chen, Liang 1 ; Cong, Yuanyuan 2 ; He, Hongxia 1 ; Yu, Ying 1 ;
作者机构： 1.Jilin Acad Agr Sci, Agrotechnol Res Inst, Changchun 130033, Peoples R China
2.Jilin Acad Agr Sci, Agr Econ & Informat Serv Ctr, Changchun 130033, Peoples R China
关键词： Agrobacterium tumefaciens;Maize transformation;Pollinated ovule culture-regeneration;GUS-expression;Zygote
期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）
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收录情况： SCI
摘要： A novel transformation system was established for maize using Agrobacterium infection of in vitro cultured ovules. The maize ovules were isolated 24 h after pollination and infected with Agrobacterium. The embryos were isolated from the pollinated ovules 2-3 weeks after Agro bacterium infection, regenerated to plantlets and investigated for transgene expression and inheritance. Experimental evaluations were focused on the four main aspects. Firstly, through the introduction of gus gene for monitoring transformation and development of embryo, it was confirmed that transgenic plants can be generated from in vitro cultured maize ovules infected with Agro bacterium. Secondly, in order to standardize the transformation protocol, several important factors that affected transformation efficiency were optimized. They included Agro bacterium delivery approach, surfactant, AS concentration, and cocultivation duration. Thirdly, stable expression and Mendelian inheritance of the introduced genes were analyzed in independent lines over two generations. Fourthly, the pollinated ovule culture-regeneration potential and transformation efficiency of five maize inbred lines were investigated to confirm the genotype independence of this transformation system. We conclude that the transformation system established in this study can be used to generate high-quality transgenic maize plants rapidly and directly
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