您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and molecular mapping of the semi-dwarf locus (sdf-1) in soybean by SLAF-seq method

文献类型： 外文期刊

作者： Dong, Zhimin 1 ; Chen, Liang 1 ; Li, Zhi 1 ; Liu, Nianxi 1 ; Zhang, Shichen 3 ; Liu, Jia 1 ; Liu, Baoquan 1 ;

作者机构： 1.Jilin Acad Agr Sci, Soybean Res Inst, Changchun 130033, Jilin, Peoples R China

2.Natl Engn Res Ctr Soybean, Changchun 130033, Jilin, Peoples R China

3.Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA

关键词： Candidate genes; High-throughput sequencing; Semi-dwarfism; SNP

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2020 年 216 卷 6 期

页码：

收录情况： SCI

摘要： Plant height is one of the most important agronomic traits in soybean. The semi-dwarf soybean cultivars could improve lodging resistance and solar radiation use efficiency so as to increase soybean yield. However, the inheritance of semi-dwarf traits has not been intensively studied and few semi-dwarf genes have been isolated in soybean. In this study, we use specific-length amplified fragment sequencing (SLAF-seq) with bulked segregant analysis (BSA) to map the semi-dwarf gene in soybean. Plant height of 213 F-2:3 families, derived from a cross between 'Jimidou-1' (semi-dwarf) and 'Gongjiao9112' (tall), was evaluated under field conditions. Genetic analysis indicated that the semi-dwarf trait was controlled by one single recessive gene. Associated with the semi-dwarf trait, SLAF-seq combined with BSA was used to develop polymorphic SLAF markers and identify a 1.04 Mb genomic region, which was designated as sdf-1. The sdf-1 locus was delimited to 80.72 kb region on chromosome 19 by KASP SNP markers developed from the SLAF markers and 6 K SNP chip. There were 14 putative genes in the associated region. From annotation information, three genes (Glyma.19g194800, Glyma.19g194500 and Glyma.19g195200) might be interesting candidate genes that are highly related to plant height growth or semi-dwarf trait. The successful identification and localization of sdf-1 could help reveal molecular mechanisms underlying soybean semi-dwarfism and to indirectly improve total yield.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台