Yeast-based assays for the high-throughput screening of inhibitors of coronavirus RNA cap guanine-N7-methyltransferase
文献类型： 外文期刊
作者： Sun, Ying 1 ; Wang, Zidao 1 ; Tao, Jiali 1 ; Wang, Yi 1 ; Wu, Andong 1 ; Yang, Ziwen 2 ; Wang, Kaimei 2 ; Shi, Liqiao 2 ; Ch 1 ;
作者机构： 1.Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Peoples R China
2.Hubei Acad Agr Sci, Biol Pesticide Engn Res Ctr, Wuhan 430072, Hubei, Peoples R China
期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The 5'-cap structure is a distinct feature of eukaryotic mRNAs and is important for RNA stability and protein translation by providing a molecular signature for the distinction of self or non-self mRNA. Eukaryotic viruses generally modify the 5'-end of their RNAs to mimic the cellular mRNA structure, thereby facilitating viral replication in host cells. However, the molecular organization and biochemical mechanisms of the viral capping apparatus typically differ from its cellular counterpart, which makes viral capping enzymes attractive targets for drug discovery. Our previous work showed that SARS coronavirus (SARS-CoV) non-structural protein 14 represents a structurally novel and unique guanine-N7-methyl-transferase (N7-MTase) that is able to functionally complement yeast cellular N7-MTase. In the present study, we developed a yeast-based system for identifying and screening inhibitors against coronavirus N7-MTase using both 96-well and 384-well microtiter plates. The MTase inhibitors previously identified by in vitro biochemical assays were tested, and some, such as sinefungin, effectively suppressed N7-MTase in the yeast system. However, other compounds, such as ATA and AdoHcy, did not exert an inhibitory effect within a cellular context. These results validated the yeast assay system for inhibitor screening yet also demonstrated the difference between cell-based and in vitro biochemical assays. The yeast system was applied to the screening of 3000 natural product extracts, and three were observed to more potently inhibit the activity of coronavirus than human N7-MTase.
- 相关文献
作者其他论文 更多>>
-
Unveiling the driving role of pH on community stability and function during lignocellulose degradation in paddy soil
作者：Wang, Yi;Chen, Yonglun;Gao, Xiuqing;Wang, Qiong;Cui, Mingyu;Guo, Peng;Chen, Yonglun;Gao, Xiuqing;Cui, Mingyu;Zhang, Dongdong;Guo, Peng
关键词：paddy soil; straw incorporation; bioaugmentation; biostimulation; microbial community; pH influence
-
Genomic and Comparative Transcriptomic Analyses Reveal Key Genes Associated with the Biosynthesis Regulation of Okaramine B in Penicillium daleae NBP-49626
作者：Wang, Yueying;Chen, Ling;Fang, Wei;Zeng, Zhen;Wu, Zhaoyuan;Liu, Fang;Liu, Xiaoyan;Gong, Yan;Zhu, Lei;Wang, Kaimei
关键词：Okaramine B; yield improvement; Penicillium daleae; complete genome; transcriptome; novel regulators; tryptophan metabolism
-
Discovery of structural diversity guided steroidal thiazolidin-4-one derivatives as potential cytotoxic agents targeting CDK1
作者：Yang, Fei;Min, Yong;Li, Kui;Yang, Ziwen;Wang, Kaimei;Gong, Yan;Liu, Manli;Ke, Shaoyong;Yang, Fei;Yang, Ziwen;Liu, Changli
关键词：Steroidal thiazolidin-4-ones; Structural diversity; Antiproliferative activity; Enzyme inhibitors; Molecular docking
-
Colonization characteristics and dynamic transition of archaea communities on polyethylene and polypropylene microplastics in the sediments of mangrove ecosystems
作者：Wang, Qiong;Zheng, Gang;Ni, Lingfang;Li, Weiye;Zheng, Daoqiong;Wu, Jiaping;Zhang, Dongdong;Wang, Qiong;Zheng, Daoqiong;Zhang, Dongdong;Wang, Qiong;Guo, Peng;Wang, Yi;Zheng, Gang;Wang, Heng;Zhang, Dongdong
关键词：Mangrove sediments; Microplastics; Archaeal biomarker; Methanogens; Assembly mechanism
-
Adaptation to appropriate sub-freezing temperatures through reduction of mycelial porosity and alteration of cell wall composition in Stropharia rugosoannulata
作者：Xue, Shujing;Pan, Siyi;Xue, Shujing;Lu, Qi;Yang, De;Guo, Peng;Wang, Yi;Wang, Qiong;Guo, Peng
关键词：Stropharia rugosoannulata; Sub-freezing temperature; Water migration; Ultrastructure modification; Cell wall
-
The viable but non-culturable (VBNC) status of Shewanella putrefaciens (S. putrefaciens) with thermosonication (TS) treatment
作者：Jiang, Ziwei;Wang, Yi;Bai, Shunjie;Bai, Chan;Li, Hailan;Guo, Peng;Liao, Tao;Qiu, Liang;Jiang, Ziwei;Wang, Yi;Bai, Shunjie;Bai, Chan;Li, Hailan;Guo, Peng;Liao, Tao;Qiu, Liang;Jiang, Ziwei;Tu, Ziyi
关键词：Shewanella putrefaciens; Thermosonication treatment; VBNC status; Protein regulation
-
Effects of humic electron mediators on reductive dechlorination of polychlorinated biphenyl by consortia enriched from terrestrial and marine environments
作者：Wang, Qiong;Wang, Yi;Guo, Peng;Wang, Qiong;Song, Wei;Zhang, Dongdong;Zhang, Dongdong;Li, Xinkai;Zhang, Dongdong;Wang, Heng;Zhang, Zhichao;Guo, Peng;Guo, Peng
关键词：PCBs; humic electron mediator; microbial reductive dichlorination; terrestrial and marine consortia; Dehalococcoides
