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Expression profiling and functional characterization of a CBL-interacting protein kinase gene from Populus euphratica

文献类型： 外文期刊

作者： Lv, Fuling 1 ; Zhang, Hechen 2 ; Xia, Xinli 1 ; Yin, Weilun 1 ;

作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China

2.Henan Acad Agr Sci, Inst Hort Res, Zhengzhou 450002, Henan, Peoples R China

关键词： Populus euphratica;SOS pathway;Salt stress;PeCIPK26

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

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收录情况： SCI

摘要： This is the first report on the function of a member of the CIPK family in Populus euphratica. The Ca2+-dependent salt overly sensitive (SOS) pathway has been shown to play an essential role in maintaining ion homeostasis and conferring salt tolerance. One component of the SOS pathway, SOS1, was identified in the salt-resistant tree P. euphratica. In this study, we identified and functionally characterized another component of the SOS pathway in this tree called PeSOS2 or PeCIPK26. On the basis of protein sequence similarity and complementation studies in Arabidopsis, PeCIPK26 was concluded to be the functional homolog of Arabidopsis AtSOS2. Yeast two-hybrid assays revealed that PeCIPK26 can interact with four calcineurin B-like (CBL) genes, i.e., PeCBL1, PeCBL4/PeSOS3, PeCBL9 and PeCBL10. Autophosphorylation assays showed that PeCIPK26 is an active protein kinase. Expression profile analysis demonstrated that PeCIPK26 is expressed in root, stem and leaf, and throughout the cell including cell membrane, cytoplasm and nucleus; in addition, it can be induced under salt-stress treatment. Functions of PeCIPK26 in salt tolerance were evaluated by gene overexpression in Arabidopsis cipk24 mutants. The better salt tolerance of transgenic plants relative to mutants was shown by their higher germination rate, lower Na+ accumulation and higher capacity to discharge Na+ when grown with NaCl. These results suggest the involvement of PeCIPK26 in the salt-stress response of P. euphratica.

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[1]The calcium sensor PeCBL1, interacting with PeCIPK24/25 and PeCIPK26, regulates Na+/K+ homeostasis in Populus euphratica. Zhang, Hechen,Lv, Fuling,Han, Xiao,Xia, Xinli,Yin, Weilun,Zhang, Hechen. 2013

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