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Functional analysis of a point mutation in the ryanodine receptor of Plutella xylostella (L.) associated with resistance to chlorantraniliprole

文献类型： 外文期刊

作者： Guo, Lei 1 ; Wang, Yi 2 ; Zhou, Xuguo 3 ; Li, Zhenyu 4 ; Liu, Shangzhong 2 ; Pei, Liang 1 ; Gao, Xiwu 1 ;

作者机构： 1.China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Sci, Beijing 100193, Peoples R China

3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA

4.Guangdong Acad Agr Sci, Inst Plant Protect, Guangzhou, Guangdong, Peoples R China

关键词： Plutella xylostella;ryanodine receptor;chlorantraniliprole;insecticide resistance;point mutation

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

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收录情况： SCI

摘要： BACKGROUND: The diamondback moth, Plutella xylostella (L.) has developed extremely high resistance to chlorantraniliprole and other diamide insecticides in the field. Aglycine to glutamic acid substitution (G4946E) in the P. xylostella ryanodine receptor (PxRyR) has been found in two resistant populations collected in Thailand and Philippines and was considered associated with the diamide insecticides resistance but no experimental evidence was provided. The present study aimed to clarify the function of the reported mutation in chlorantraniliprole resistance in P. xylostella. RESULTS: We identified the same mutation (G4946E) in PxRyR from four field collected chlorantraniliprole resistant populations of Plutella xylostella in China. Most importantly, we found that the frequency of the G4946E mutation is significantly correlated to the chlorantraniliprole resistance ratios in P. xylostella (R~2 = 0.82, P = 0.0003). Ligand binding assays showed that the binding affinities of the PxRyR to the chlorantraniliprole in three field resistant populations were 2.41 -, 2.54- and 2.60-times lower than that in the susceptible one. CONCLUSION: For the first time we experimentally proved that the G4946E mutation in PxRyR confers resistance to chlorantraniliprole in Plutella xylostella. These findings pave the way for the complete understanding of the mechanisms of diamide insecticides resistance in insects.

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