文献类型： 外文期刊
作者： Yang, Liyan 1 ; Sun, Yi 2 ; Wang, Yixue 2 ; Hao, Yaoshan 2 ;
作者机构： 1.Shanxi Normal Univ, Dept Life Sci, Linfen 041004, Peoples R China
2.Shanxi Acad Agr Sci, Biotechnol Res Ctr, Taiyuan 030031, Peoples R China
3.Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Taiyuan 030031, Peoples R China
关键词： transgenic poplar;feed;rabbit;biosafety
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.638； 五年影响因子：3.802 ）
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收录情况： SCI
摘要： BACKGROUND Various studies have been conducted to evaluate the effect of Bt crops on animals. Insect-resistant bioassays have revealed that the chitinase-BmkIT combination could be used as a new pest-resistant gene source and might be a complementary alien gene source to the Bt toxin gene. So it is necessary to assay the effects of chitinase-BmkIT plants on animals. RESULTS Forty 40-day old New Zealand White Rabbits (Oryctolagus cuniculus) were fed for 60 consecutive days with formulated feed containing dehydrated poplar (Populus cathayana Rehd) leaves harbouring chitinase-BmkIT gene combination or untransformed counterparts, and the potential toxicological effects of transgenic leaves on rabbits were explored. The results of the growth study revealed no significant differences for daily weight gain, feed intake and feed conversion ratio where they were 101.6%, 99.2% and 97.8% of the treatment compared to the control, respectively. No obvious pathological change was observed in the small intestine, stomach, spleen, kidney, lung, heart, bladder, pancreas, prostate and ovary. Electron microscopy observations of liver cells and renal cells showed they were both normal in the two groups. No feed-derived chitinase, BmkIT and NPTII genes were found in small intestine, blood, or leg muscle samples although they were detected in the formulated feed. CONCLUSION We conclude that the processed poplar leaves with foreign chitinase-BmkIT genes had no obviously harmful effects on rabbits
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