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cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda

文献类型： 外文期刊

作者： Duan, Yafei 1 ; Liu, Ping 1 ; Li, Jitao 1 ; Li, Jian 1 ; Gao, Baoquan 1 ; Chen, Ping 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, Guangzhou 510300, Guangdong, People

关键词： Exopalaemon carinicauda;Peroxiredoxin 5 (Prx5);Gene cloning;Expression

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

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收录情况： SCI

摘要： Peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species. In this study, a full-length of peroxiredoxin 5 (designated EcPrx5) cDNA was cloned from the ridgetail white prawn Exopalaemon carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of the EcPrx5 was of 827 bp, containing a 5' untranslated region (UTR) of 14 bp, a 3' UTR of 228 bp with a poly (A) tail, and an open reading frame of 585 bp encoding a polypeptide of 194 amino acids with the predicted molecular weight of 20.83 kDa and estimated isoelectric point of 7.62. BLAST analysis revealed that amino acids of EcPrx5 shared 89, 68, 66, 65, 53 and 51 % identity with that of Macrobrachium rosenbergii, Megachile rotundata, Harpegnathos saltator, Acromyrmex echinatior, Danio rerio, and Homo sapiens counterparts, respectively. The conserved Prx domain and the signature of peroxiredoxin catalytic center identified in EcPrx5 suggested that EcPrx5 belonged to the atypical 2-Cys Prx subgroup. Real time quantitative RT-PCR analysis indicated that EcPrx5 could be detected in all the tested tissues with highest expression level in hepatopancreas. As time progressed, the expression level of EcPrx5 both in hemocytes and hepatopancreas increased in the first 6 h after Vibrio anguillarum and white spot syndrome virus challenge, and showed different expression profiles. The results indicated that EcPrx5 involved in immune response against bacterial and viral infection in E. carinicauda.

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[1]Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei.

[2]Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun. 2014

[3]A farnesoic acid O-methyltransferase (FAMeT) from Exopalaemon carinicauda is responsive to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[4]Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress. Ren Hai,Wu Jianhua,Ren Hai,Li Jian,Li Jitao,Liu Ping,Liang Zhongxiu. 2015

[5]Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress. Ren Hai,Ren Hai,Li Jian,Li Jitao,Ying Yu,Ge Hongxing,Li Dongli,Yu Tianji. 2015

[6]The roles of Na+/K+-ATPase alpha-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses. Li, Jitao,Ma, Peng,Liu, Ping,Chen, Ping,Li, Jian.

[7]Cloning of a heat shock protein 90 (HSP90) gene and expression analysis in the ridgetail white prawn Exopalaemon carinicauda. Li, Jitao,Han, Junying,Chen, Ping,Chang, Zhigjang,He, Yuying,Liu, Ping,Wang, Qingyin,Li, Jian. 2012

[8]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[9]Molecular cloning and gene expression analysis of cystatin C-like proteins in spinyhead croaker Collichthys lucidus. Song, W.,Jiang, K. J.,Zhang, F. Y.,Zhao, M.,Ma, L. B.. 2016

[10]Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei. 2013

[11]Isolation of Prawn (Exopalaemon carinicauda) Lipopolysaccharide and beta-1, 3-Glucan Binding Protein Gene and Its Expression in Responding to Bacterial and Viral Infections. Ge Qianqian,Li Jitao,Sun Ming,Ge Qianqian,Li Jian,Sun Ming,Zhao Fazhen,Ge Qianqian,Li Jian,Sun Ming,Zhao Fazhen,Duan Yafei. 2016

[12]Molecular characterization and expression analysis of the beta-actin gene from the ridgetail white prawn Exopalaemon carinicauda. Liang, J. P.,Nie, G. X.,Liang, J. P.,Ge, Q. Q.,Li, J. T.,Liu, P.,Li, J.,Wang, Y.. 2016

[13]Transcriptome analysis of eyestalk and hemocytes in the ridgetail white prawn Exopalaemon carinicauda: assembly, Annotation and Marker Discovery. Li, Jitao,Li, Jian,Chen, Ping,Liu, Ping,He, Yuying.

[14]cDNA cloning, characterization and expression analysis of ATP-binding cassette transmembrane transporter in Exopalaemon carinicauda. Zhai, Qian-Qian,Li, Jian,Chang, Zhi-Qiang,Zhai, Qian-Qian,Li, Jian,Chang, Zhi-Qiang.

[15]The responsive expression of a chitinase gene in the ridgetail white prawn Exopalaemon carinicauda against Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[16]Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda. Jia, S. W.,Liu, P.,Li, J.,Li, J. T.,Jia, S. W.,Pan, L. Q.. 2013

[17]Transcriptome analysis of the hepatopancreas in Exopalaemon carinicauda infected with an AHPND-causing strain of Vibrio parahaemolyticus. Ge, Qianqian,Li, Jian,Wang, Jiajia,Li, Jitao,Ge, Hongxing,Zhai, Qianqian,Ge, Qianqian,Li, Jian,Wang, Jiajia,Li, Jitao,Ge, Hongxing,Zhai, Qianqian.

[18]Comparison of White Spot Syndrome Virus Infection Resistance Between Exopalaemon carinicauda and Litopenaeus vannamei Under Different Salinity Stresses. Ge Qianqian,Yu Ge,Sun Ming,Li Jitao,Li Jian,Ge Qianqian,Li Jitao,Li Jian. 2017

[19]The role of oncoprotein NM23 gene from Exopalaemon carinicauda is response to pathogens challenge and ammonia-N stress. Duan, Yafei,Zhang, Zhe,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping.

[20]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

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