文献类型： 外文期刊

作者： Beloglazova, N. V. ¹ ; Speranskaya, E. S. ¹ ; Wu, A. ² ; Wang, Z. ³ ; Sanders, M. ¹ ; Goftman, V. V. ¹ ; Zhang, D. ⁴ ; Go ¹ ;

作者机构： 1.Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai 201403, Peoples R China

3.China Agr Univ, Natl Ctr Vet Drug Safety Evaluat, Coll Vet Med, Beijing 100094, Peoples R China

4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China

5.Saratov NG Chernyshevskii State Univ, Fac Chem, Dept Gen & Inorgan Chem, Saratov 410012, Russia

关键词： Multiassay;Immunoassay;Quantum dots;Mycotoxin;Rapid tests;Multiplex screening

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

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收录情况： SCI

摘要： The aim of this manuscript was the development of easy-to-operate quantum dots (QDs)-based immunochemical techniques for simultaneous screening of several mycotoxins in cereals. Two different approaches for multiplex fluorescent immunosorbent assay (FLISA) were employed. In the first approach a multiwell plate in which the different wells express a different mycotoxin (deoxynivalenol, zearale-none, aflatoxin B1, T2-toxin and fumonisin B1) was considered as a multiplex because each sample was pretreated once and then will be distributed over a series of wells within the same plate (single-analyte multiplex, SAM). The entire assay allows the simultaneous determination of all compounds. For the double-analyte multiplex (DAM) two different specific antibodies were co-immobilized in one single well. Zearalenone and aflatoxin B1 were simultaneously determined, provided their conjugates are labeled with QDs which are fluorescent in different parts of the spectrum, by scanning the assay outcome at two different wavelengths. The limits of detection (LOD) for the simultaneous determination of deoxynivalenol, zearalenone, aflatoxin B1, T2-toxin and fumonisin B1 by SAM FLISA were 3.2,0.6,0.2,10 and 0.4 μg kg ~(-1), respectively, while for the DAM FLISA they were 1.8 and 1 μg kg ~(-1) for zearalenone and aflatoxin B1, respectively. SAM FLISA principle was also presented in a qualitative on-site format and tested for on-site multiplex determination of four mycotoxins in cereals. The achieved cut-off values of 500, 100, 2 and 100 μg kg~(-1) for deoxynivalenol, zearalenone, aflatoxin B1 and T2-toxin respectively. For simplification of multiassay results, evaluation the conjugates with QDs of different colors were used.