您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid detection of mud crab dicistrovirus-1 using loop-mediated isothermal amplification

文献类型： 外文期刊

作者： Guo, Zhixun 1 ; Zhang, Di 1 ; Ma, Hongling 1 ; Su, Youlu 1 ; Feng, Juan 1 ; Xu, Liwen 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Scylla paramamosain;Mud crab dicistrovirus-1;Loop-mediated isothermal amplification

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mud crab dicistrovirus-1 (MCDV-1) was isolated from the mud crab (Scylla paramamosain), resulting in mass mortality and widespread economic loss in China. In this study, a detection method for MCDV-1 using loop-mediated isothermal amplification was developed. Two pairs of primers targeting the VP2 gene were designed. These primers were the outer primers F3 and B3, and the inner primers FIP and BIP. Optimal amplification was carried out using 0.2 mu mol/L F3/B3, 1.6 mu mol/L FIP/BIP, 6 mmol/L Mg2+, 0.8 mmol/L dNTPs, and 0.8 mol/L betaine, and completed in 1 h at 62 degrees C. The products demonstrated a ladder pattern on agarose gel electrophoresis and could also be detected visually according to turbidity, or by adding SYBR Green I and observing a color change from orange to green. The proposed method could specifically amplify MCDV-1 gene fragments. Sensitivity assay revealed that six copies of the viral genome could be detected by this method, which was 1000-fold more sensitive than that of conventional PCR using constructed plasmid as amplification template. At clinical sample level, sensitivity of LAMP was 100-fold higher than that of conventional PCR

  • 相关文献

[1]Pathogenicity and complete genome sequence analysis of the mud crab dicistrovirus-1. Guo, Zhi-Xun,He, Jian-Guo,Weng, Shao-Ping,He, Jian-Guo,Xu, Hai-Dong,Guo, Zhi-Xun.

[2]Characterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain. Wang, J.,Jiang, K. J.,Zhang, F. Y.,Song, W.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.,Ma, L. B.,Wang, J.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.. 2015

[3]Multiplex immune-related genes expression analysis response to bacterial challenge in mud crab, Scylla paramamosain. Zhang, Fengying,Jiang, Keji,Sun, Manman,Zhang, Dan,Ma, Lingbo. 2013

[4]Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain. Zhu, Houze,Pi, Yan,Jiang, Keji,Zhang, Fengying,Zhang, Dan,Ma, Lingbo,Zhang, Dan. 2012

[5]Identification of a trypsin gene from Scylla paramamosain and its expression profiling during larval development. Jiang, Keji,Zhang, Fengying,Zhang, Dan,Zhang, Yong,Qiao, Zhenguo,Ma, Lingbo,Tao, Qichang,Pi, Yan. 2011

[6]Correlation of growth-related traits and their effects on body weight of the mud crab (Scylla paramamosain). Ma, H. Y.,Ma, C. Y.,Ma, L. B.,Xu, Z.,Feng, N. N.,Qiao, Z. G.. 2013

[7]The complete mitochondrial genome sequence and gene organization of the mud crab (Scylla paramamosain) with phylogenetic consideration. Ma, Hongyu,Ma, Chunyan,Li, Xincang,Xu, Zhen,Feng, Nana,Ma, Lingbo.

[8]Population genetic diversity of mud crab (Scylla paramamosain) in Hainan Island of China based on mitochondrial DNA. Ma, Hongyu,Ma, Chunyan,Ma, Lingbo.

[9]Parentage assignment of the mud crab (Scylla paramamosain) based on microsatellite markers. Ma, Qunqun,Ma, Hongyu,Ma, Chunyan,Feng, Nana,Xu, Zhen,Li, Shujuan,Jiang, Wei,Qiao, Zhenguo,Ma, Lingbo,Ma, Qunqun,Ma, Hongyu,Chen, Jianhua.

[10]A novel anti-lipopolysaccharide factor SpALF6 in mud crab Scylla paramamosain exhibiting different antimicrobial activity from its single amino acid mutant. Hou, Zhi-Guo,Wang, Yuan,Fang, Wen-Hong,Zhao, Shu,Zhang, Jing-Xiao,Li, Xin-Cang,Hou, Zhi-Guo,Hui, Kaimin,Ma, Hongyu.

[11]Two transcripts of HMG-CoA reductase related with developmental regulation from Scylla paramamosain: Evidences from cDNA cloning and expression analysis. Zhao, Ming,Jiang, Keji,Song, Wei,Ma, Chunyan,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Qiao, Zhenguo,Zhang, Fengying,Ma, Lingbo,Zhao, Ming,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Chen, Kai.

[12]Amino Acid, Fatty Acid, and Metal Compositions in Edible Parts of Three Cultured Economic Crabs: Scylla paramamosain, Portunus trituberculatus, and Eriocheir sinensis. Jiang, Ke-Ji,Zhang, Feng-Ying,Zhang, Dan,Sun, Man-Man,Gao, Lu-Jiao,Qiao, Zhen-Guo,Ma, Ling-Bo,Pi, Yan,Jiang, Li-Li,Yu, Zhong-Li,Zhang, Dan,Sun, Man-Man.

[13]Characteristics of growth traits and their effects on body weight of G(1) individuals in the mud crab (Scylla paramamosain). Jiang, W.,Ma, H. Y.,Ma, C. Y.,Li, S. J.,Liu, Y. X.,Qiao, Z. G.,Ma, L. B.,Jiang, W.. 2014

[14]Isolation and characterization of polymorphic microsatellite loci from cDNA library of Scylla paramamosain. Ma, Chunyan,Ma, Hongyu,Ma, Lingbo,Jiang, Keji,Zhang, Fengying,Song, Wei. 2011

[15]Late Pleistocene population expansion of Scylla paramamosain along the coast of China: A population dynamic response to the Last Interglacial sea level highstand. He, Lijun,Jiang, Chaojun,Qiao, Zhenguo,Zhang, Aibing,Weese, David,Zhu, Chaodong.

[16]Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain). Yang, Xiaolong,Lin, Fan,Wu, Qingyang,Zhang, Yueling,You, Cuihong,Liu, Wenhua,Cheng, Yinwei,Shi, Xi,Wang, Shuqi,Ma, Hongyu,Ikhwanuddin, Mhd,Li, Xincang. 2018

[17]Characterization of hemocyanin from the mud crab Scylla paramamosain and its expression analysis in different tissues, at various stages, and under Vibrio parahaemolyticus infection. Wang, J.,Zhang, F. Y.,Song, W.,Hu, J. H.,Zhao, M.,Jiang, K. J.,Ma, L. B.,Wang, J.,Hu, J. H.,Zhao, M.,Fang, Y. B.. 2015

[18]Macrophage Migration Inhibitory Factor in Mud Crab Scylla paramamosain: Molecular Cloning, Expression Profiles in Various Tissues and under Vibrio challenge. Fang, Y.,Jiang, K.,Zhang, F.,Sun, M.,Hu, J.,Ma, L.,Fang, Y.,Sun, M.,Hu, J..

[19]Isolation and Characterization of a Ferritin cDNA from the Mud Crab Scylla paramamosain. Zhang, Dan,Jiang, Keji,Zhang, Fengying,Ma, Chunyan,Shi, Yanhong,Qiao, Zhenguo,Ma, Lingbo,Zhang, Dan.

[20]High genetic diversity and low differentiation in mud crab (Scylla paramamosain) along the southeastern coast of China revealed by microsatellite markers. Ma, Hongyu,Cui, Haiyu,Ma, Chunyan,Ma, Lingbo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台